PXD010778

PXD010778 is an original dataset announced via ProteomeXchange.

Title	Influence of International Space Station Conditions on Fungal ‘Omics’ in Aspergillus nidulans
Description	This study presents the first global genomic, proteomic, and secondary metabolomic characterization of the filamentous fungus, Aspergillus nidulans, following growth on the International Space Station (ISS). The investigation included the A. nidulans wild-type and 3 mutant strains, two of which were genetically engineered to enhance secondary metabolite (SM) production. Whole genome sequencing (WGS) revealed that ISS conditions altered the A. nidulans genome in specific regions. In strain CW12001, which features overexpression of the SM global regulator laeA, ISS conditions induced a point mutation that resulted in the loss of the laeA stop codon. Differential expression of proteins involved in stress response, carbohydrate metabolic processes, and SM biosynthesis was observed. ISS conditions significantly decreased prenyl xanthone production in the wild-type strain and increased asperthecin production in LO1362 and CW12001, which are deficient in a major DNA repair mechanism. Together, these data provide valuable insights into the genetic and molecular adaptation mechanism of A. nidulans to the spacecraft environment and present many economic benefits.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_06:42:56.530.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Abby Chiang
SpeciesList	scientific name: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans); NCBI TaxID: 227321;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-15 02:46:04	ID requested
⏵ 1	2022-05-19 06:42:57	announced

Romsdahl J, Blachowicz A, Chiang AJ, Chiang YM, Masonjones S, Yaegashi J, Countryman S, Karouia F, Kalkum M, Stajich JE, Venkateswaran K, Wang CCC, International Space Station conditions alter genomics, proteomics, and metabolomics in Aspergillus nidulans. Appl Microbiol Biotechnol, 103(3):1363-1377(2019) [pubmed]

curator keyword: Biological

submitter keyword: Aspergillus nidulans, International Space Station, Omics

Markus Kalkum
contact affiliation	Beckman Research Institute of City of Hope
contact email	mkalkum@coh.org
lab head
Abby Chiang
contact affiliation	City of Hope
contact email	jchiang@coh.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010778
     1. Label: PRIDE project
     2. Name: Influence of International Space Station Conditions on Fungal ‘Omics’ in Aspergillus nidulans