PXD010773

PXD010773 is an original dataset announced via ProteomeXchange.

Title	An interaction network of the human SEPT9 established by quantitative mass spectrometry reveals an interplay with myosin motors
Description	Experimentally deciphering and understanding the interaction network of a particular protein provides often evidence for so far unknown functions. For the septins, a class of cytoskeletal proteins, targeted high-throughput approaches that aim at systematically deciphering interaction partners have not yet been performed. Septins regulate the organization of the acin cytoskeleton, vesicle transport and fusion, chromosome alignment- and segregation, and cytokinesis. SEPT9 is part of the core septin hetero-octamer in human cells which is composed of SEPT2, SEPT6, SEPT7, and SEPT9. SEPT9 has been linked to a variety of intracellular functions as well as to diseases and diverse types of cancer. We applied a quantitative proteomics approach to establish an interactome of SEPT9 in human fibroblast cells. We identified among others so far unknown interaction partners from the myosin familiy and could provide evidence that SEPT9 participates in vesicle transport from and to the plasma membrane as well as in the attachment of actin stress fibers to cellular adhesions.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_13:04:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastian Wiese
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-14 07:40:59	ID requested
⏵ 1	2019-11-12 13:04:36	announced

Hecht M, R, ö, sler R, Wiese S, Johnsson N, Gronemeyer T, An Interaction Network of the Human SEPT9 Established by Quantitative Mass Spectrometry. G3 (Bethesda), 9(6):1869-1880(2019) [pubmed]

curator keyword: Biological

submitter keyword: Septin, Human, AP-MS, Myosin

Sebastian Wiese
contact affiliation	Core Unit Mass Spectrometry and Proteomics Medical faculty Ulm University Germany
contact email	sebastian.wiese@uni-ulm.de
lab head
Sebastian Wiese
contact affiliation	Universität Ulm
contact email	sebastian.wiese@uni-ulm.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD010773

PRIDE project URI

• PRIDE
  1. PXD010773
     1. Label: PRIDE project
     2. Name: An interaction network of the human SEPT9 established by quantitative mass spectrometry reveals an interplay with myosin motors