PXD010772

PXD010772 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of immunocaptured mitochondria from Purkinje Cells
Description	Mitochondria vary in morphology and function in different tissues, however little is known about their molecular diversity among cell types. To investigate mitochondrial diversity in vivo, we developed an efficient protocol to isolate cell type-specific mitochondria based on a new MitoTag mouse. We profiled the mitochondrial proteome of three major neural cell types in cerebellum and identified a substantial number of differential mitochondrial markers for these cell types in mice and humans. Based on predictions from these proteomes, we demonstrate that astrocytic mitochondria metabolize long-chain fatty acids more efficiently than neurons. Moreover, we identified Rmdn3 as a major determinant of ER-mitochondria proximity in Purkinje cells. Our novel approach enables exploring mitochondrial diversity on the functional and molecular level in many in vivo contexts.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:54:42.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-14 06:55:22	ID requested
1	2019-09-03 01:43:47	announced
⏵ 2	2024-10-22 04:54:43	announced	2024-10-22: Updated project metadata.

10.1038/s41593-019-0479-z;

Fecher C, Trov, ò L, M, ü, ller SA, Snaidero N, Wettmarshausen J, Heink S, Ortiz O, Wagner I, K, ü, hn R, Hartmann J, Karl RM, Konnerth A, Korn T, Wurst W, Merkler D, Lichtenthaler SF, Perocchi F, Misgeld T, Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity. Nat Neurosci, 22(10):1731-1742(2019) [pubmed]

ProteomeXchange project tag: Mitochondria (HPP)

curator keyword: Biological

submitter keyword: Mitochondria,Purkinje Cells, Neurons

Thomas Misgeld
contact affiliation	Institute of Neuronal Cell Biology Technical University of Munich Germany
contact email	thomas.misgeld@tum.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD010772

PRIDE project URI

• PRIDE
  1. PXD010772
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of immunocaptured mitochondria from Purkinje Cells