PXD010768

PXD010768 is an original dataset announced via ProteomeXchange.

Title	Trypanosoma cruzi LC-MSMS - Trypanosoma cruzi, the etiologic agent of Chagas disease, cycles through different life stages characterized by defined molecular traits associated with the proliferative or differentiation state
Description	Trypanosoma cruzi, the etiologic agent of Chagas disease, cycles through different life stages characterized by defined molecular traits associated with the proliferative or differentiation state. In particular, T. cruzi epimastigotes are the replicative forms that colonize the intestine of the Triatomine insect vector before entering the stationary phase that is crucial for differentiation into metacyclic trypomastigotes, which are the infective forms of mammalian hosts. The transition from proliferative exponential phase to quiescent stationary phase represents an important step that recapitulates the early molecular events of metacyclogenesis, opening new possibilities for understanding this process. In this study, we report a quantitative shotgun proteomic analysis of the T. cruzi epimastigote in the exponential and stationary growth phases. More than 3000 proteins were detected and quantified, highlighting the regulation of proteins involved in different subcellular compartments. Ribosomal proteins were upregulated in the exponential phase, supporting the higher replication rate of this growth phase. Autophagy-related proteins were upregulated in the stationary growth phase, indicating the onset of the metacyclogenesis process. Moreover, this study reports the regulation of N-terminally acetylated proteins during growth phase transitioning, adding a new layer of regulation to this process. Taken together, this study reports a proteome-wide rewiring during T. cruzi transit from the replicative exponential phase to the stationary growth phase, which is the preparatory phase for differentiation
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:47:44.519.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simon Mule
SpeciesList	scientific name: Trypanosoma cruzi cruzi; NCBI TaxID: 85057;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-14 02:30:49	ID requested
1	2018-08-22 02:16:09	announced
2	2018-12-04 03:57:27	announced	Updated project metadata.
3	2020-03-13 01:33:00	announced	2020-03-13: Updated project metadata.
⏵ 4	2024-10-22 04:47:44	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Trypanosoma cruzi

metacyclogenesis

exponential and stationary phases

quantitative proteomics

Gim5a

N-acetyltransferases

protein N-terminal acetylation

autophagy

Simon Mule
contact affiliation	Glycoproteomics Laboratory, Department of Parasitology, Institute of Biomedical Sciences, Universidade of Sao Paulo
contact email	ngaosimon@gmail.com
lab head
Simon Mule
contact affiliation	Universidade de São Paulo
contact email	ngaosimon@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010768

PRIDE project URI

• PRIDE
  1. PXD010768
     1. Label: PRIDE project
     2. Name: Trypanosoma cruzi LC-MSMS - Trypanosoma cruzi, the etiologic agent of Chagas disease, cycles through different life stages characterized by defined molecular traits associated with the proliferative or differentiation state