PXD010762

PXD010762 is an original dataset announced via ProteomeXchange.

Title	comparative proteome analysis of serum exosomes from newborn piglets inresponse to porcine epidemic diarrhea virus infection
Description	The immune system is thought to be fragile in the neonate, which is susceptible to pathogens. Exosomes are a type of vehicles existing in the body fluid and participate in many biological processes, especially the immune response. Inorder to investigate the roles that exosomes may play during virus infection in the neonate, porcine epidemic diarrhea virus (PEDV), a devastating enteric virus to newborn piglets, was selected for infection. Serum exosomes were then isolated from the newborn-piglets infected or mock-infected with PEDV and followed by a label-free LC-MS/MS based comparative quantitative proteomic analysis. Among 441 proteins detected in the serum exosomes, there were lots of complement proteins. The expression level of the complement C3, C6 and CFB suffered drastic changes due to PEDV infection. After the confirmation by western-blot assay, we then investigated the function of these exosomes on PEDV infection and discovered that the exosomes from mock-infected newborn piglets restricted PEDV infection but this inhibition disappeared after exosomes were heat-inactivated, suggesting that the complement is one of the key antiviral molecules. These findings will facilitate the understanding of the antiviral response of the neonate mediated by exosomes
HostingRepository	PRIDE
AnnounceDate	2019-04-30
AnnouncementXML	Submission_2019-05-02_05:25:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	jianing chen
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-13 08:23:10	ID requested
1	2019-04-30 11:25:01	announced
⏵ 2	2019-05-02 05:38:53	announced	Updated publication reference for PubMed record(s): 30983354.

Chen J, Jin L, Yan M, Yang Z, Wang H, Geng S, Gong Z, Liu G, Serum Exosomes from Newborn Piglets Restrict Porcine Epidemic Diarrhea Virus Infection. J Proteome Res, 18(5):1939-1947(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Serum exosome, neonate, complement, porcine epidemic diarrhea virus, antiviral effect

liu guangliang
contact affiliation	animal immunology group, lanzhou veterinary research institute
contact email	liuguangliang01@caas.cn
lab head
jianing chen
contact affiliation	lanzhou veterinary research institute
contact email	chenjianing@caas.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010762
     1. Label: PRIDE project
     2. Name: comparative proteome analysis of serum exosomes from newborn piglets inresponse to porcine epidemic diarrhea virus infection