PXD010757

PXD010757 is an original dataset announced via ProteomeXchange.

Title	Proteomics reveals ablation of PlGF down-regulate the insulin resistance pathway, increase antioxidant and neuroprotective proteins in the diabetic mice retina
Description	PGF (Placental growth factor) is a member of the vascular endothelial growth factor (VEGF) sub-family a crucial factor in angiogenesis and vasculogenesis. Mechanisms by which PlGF expression is regulated remain to be investigated in diabetic retinopathy DR. However, the underlying molecular mechanisms that PlGF, mediates in the early complications at non-proliferative DR remain mostly elusive. Here we have applied an LC-MS/MS-based label-free quantification proteomic approach to PlGF ablation in the retinal tissues from mouse strains (Akita, PlGF-/- and Akita.PlGF-/-). The proteomes of retinal tissues were extracted, digested by the Trypsin/LysC enzyme and subsequently analyzed by a Q-Exactive hybrid Quadrupole-Orbitrap mass spectrometer. We have performed normalization by the Z-score method, correlation by Pearson correlation coefficient and identified differentially expressed proteins in four comparisons. The gene ontology, functional pathways, and protein-protein network interaction analysis suggested that Gnb1, Gnb2, Gnb4, Gnai2, Gnao1, Snap2 and Gngt1 proteins are involved in insulin resistance pathways, which are down-regulated/ no significant in PlGF ablation in Akita diabetics (Akita.PlGF-/- vs. Akita), up-regulated in Akita vs. C57, PlGF-/- vs. C57. Prdx6, Map2 are involved in antioxidant activity and neural protection pathways respectively, which are up-regulated in Akita.PlGF-/- vs. Akita. Our proteomics outcomes anticipated that down-regulated of insulin resistance pathways, up-regulation of antioxidant and neuroprotection proteins might epitomize the potential mechanisms of anti-PlGF therapies in the treatment of DR.
HostingRepository	PRIDE
AnnounceDate	2019-03-13
AnnouncementXML	Submission_2019-03-13_03:05:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Madhu Saddala Saddala
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-13 06:32:01	ID requested
⏵ 1	2019-03-13 03:05:19	announced

Saddala MS, Lennikov A, Grab DJ, Liu GS, Tang S, Huang H, Proteomics reveals ablation of PlGF increases antioxidant and neuroprotective proteins in the diabetic mouse retina. Sci Rep, 8(1):16728(2018) [pubmed]

ProteomeXchange project tag: Diabetes (B/D-HPP)

curator keyword: Biological, Biomedical

submitter keyword: Diabetes, Akita mice, PlGF-knockout, Mass spectrometry, proteomics

Dr. Hu Huang
contact affiliation	Wilmer Eye Institute, Johns Hopkins University, Baltimore, Maryland, United States of America
contact email	hhuang27@jhmi.edu
lab head
Madhu Saddala Saddala
contact affiliation	Johns Hopkins University School of Medicine
contact email	madhubioinformatics@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010757
     1. Label: PRIDE project
     2. Name: Proteomics reveals ablation of PlGF down-regulate the insulin resistance pathway, increase antioxidant and neuroprotective proteins in the diabetic mice retina