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PXD010753

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-05-15
  • AnnouncementXML: Submission_2019-05-15_00:46:21.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Keshava Prasad T. S.
  • Title: Proteomics of Asrij perturbed Drosophila lymph glands as a resource for identification of new regulators of hematopoiesis
  • Description: Hematological disorders result in perturbed homeostasis of the blood system. However, a comprehensive understanding of how physiological and genetic mechanisms regulate blood cell precursor maintenance and differentiation is lacking. Owing to simplicity and ease of genetic analysis, the Drosophila melanogaster lymph gland (LG) is an excellent model to study hematopoiesis. The LG is a multi-lobed structure compartmentalized into precursor and differentiation zones whose geography and identity is regulated by multiple signalling pathways. While additional molecular and functional subtypes are expected there is a paucity of information on gene expression and regulation of hemocyte homeostasis. Hence, we quantitatively analyzed the LG proteome under conditions that maintain precursors or promote their differentiation in vivo, by perturbing expression of Asrij, a conserved endosomal regulator of hematopoiesis. Although technically demanding, we pooled samples obtained from 1500 larval dissections per genotype and using iTRAQ quantitative proteomics, determined the relative expression levels of polypeptides in Asrij knockout (KO) and overexpressing (OV) LGs in comparison to wild type (control). Mass spectrometry data analysis showed that at least 6.5% of the Drosophila proteome is expressed in wild type LGs.Of 2,133 proteins identified, 780 and 208 proteins were common to the previously reported cardiac tube and hemolymph proteomes, respectively, resulting in the identification of 1238 proteins exclusive to the LG. Perturbation of Asrij levels led to differential expression of 619 proteins, of which 23% have human homologs implicated in various diseases. Proteins regulating metabolism, immune system, signal transduction and vesicle-mediated transport were significantly enriched. Immunostaining of representative candidates from the enriched categories and previous reports confirmed 75% of our results and validated the LG proteome. Our study provides, for the first time, an in vivo proteomics resource for identifying novel regulators of hematopoiesis that will also be applicable to understanding vertebrate blood cell development.
  • SpeciesList: scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
  • ModificationList: iTRAQ4plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap Velos

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-08-10 06:33:43ID requested
12019-05-15 00:46:22announced

Publication List

  1. Dataset with its publication pending

Keyword List

  1. curator keyword: Biological, Biomedical
  2. submitter keyword: Drosophila hematopoiesis, proteome, lymph gland, Asrij, ESCRT, differential regulation

Contact List

    Keshava Prasad
    • contact affiliation: Institute of Bioinformatics, International Technology Park, Bangalore 560066
    • contact email: keshav@ibioinformatics.org
    • lab head:
    Keshava Prasad T. S.
    • contact affiliation: Institute of Bioinformatics, Bangalore, Karnataka, India.
    • contact email: keshav@ibioinformatics.org
    • dataset submitter:

Full Dataset Link List

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  2. PRIDE project URI
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