PXD010751

PXD010751 is an original dataset announced via ProteomeXchange.

Title	Nitric oxide modulates Saccharomyces cerevisiae biofilm formation though regulating the transmembrane protein CTR1 by activating its transcriptional factor MAC1.
Description	Biofilms with immobilized cells in industrial fermentation are beneficial. Encased in extracellular polymeric substance, cells forming biofilms are regulated by various factors. Nitric oxide (NO), as a signaling molecule, recognized as quorum sensing molecule regulating microbe biofilm formation. Regulation mechanisms of NO on bacteria biofilm have been studied extensively and deeply, while on fungus are rarely studied. In this study, we observed that low concentration of NO enhanced S. cerevisiae biofilm formation. Transcriptional and proteomic analysis revealed that transcription factor MAC1 was activated in biofilm cells under NO treatment. Overexpressed MAC1 increased yeast biofilm formation bypassing regulating the expression level of FLO11. Increased copper and iron contents in NO treated and MAC1 overexpressed cells were not responsible for increased biofilm formation. Among six downstream genes of MAC1, overexpressed CTR1 contributed yeast biofilm formation. Moreover, MAC1 and CTR1 contributed to biofilm cells ethanol resistance resulting from enhanced biofilm. The role of CTR1 protein in yeast biofilm formation may result from its hydrophobic residues in N-terminal extracellular domain. These findings suggested a NO-mediated biofilm formation mechanism that NO regulated expression levels of CTR1 through activating its transcription factor MAC1, leading enhanced biofilm formation.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_12:54:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaomei li
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-10 06:33:10	ID requested
⏵ 1	2019-11-12 12:54:45	announced

Yang L, Zheng C, Chen Y, Shi X, Ying Z, Ying H, by activating the transcriptional factor Mac1p and thereby regulating the transmembrane protein Ctr1. Biotechnol Biofuels, 12():30(2019) [pubmed]

curator keyword: Biological

submitter keyword: Saccharomyces cerevisiae, Nitric oxide, biofilm, Transcription factor MAC1

Hanjie Ying
contact affiliation	State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, P.R. China;
contact email	yinghanjie@njtech.edu.cn
lab head
xiaomei li
contact affiliation	PTM Biolabs lnc.
contact email	xiaomei_li@ptm-biolab.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010751
     1. Label: PRIDE project
     2. Name: Nitric oxide modulates Saccharomyces cerevisiae biofilm formation though regulating the transmembrane protein CTR1 by activating its transcriptional factor MAC1.