PXD010747 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SWATH proteomics reveals that reductants selectively disrupt collagen homeostasis in human dermal fibroblasts and modify growth factor-independent signalling through the MAPK/Akt pathway |
Description | We have used an unbiased, label-free SWATH proteomic approach to quantitate the response of skin fibroblast cells to the reductant DTT in the presence or absence of the growth factor PDGF. Of the 4487 proteins that were identified, only 47 proteins showed a statistically significant change of 2-fold or more with reducing stress. Our data demonstrates that reducing stress results in the loss of a small subset of reductant-sensitive proteins, including the fibrillar collagens (COL1A1/2 and COL3A1) and the myopathy-associated bundling collagen COL6A1/2/3. |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-25 |
AnnouncementXML | Submission_2019-03-25_04:52:30.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adrian Brown |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-10 02:28:08 | ID requested | |
⏵ 1 | 2019-03-25 04:52:31 | announced | |
Publication List
Carne NA, Bell S, Brown AP, M, ä, ä, tt, ä A, Flagler MJ, Benham AM, Reductive Stress Selectively Disrupts Collagen Homeostasis and Modifies Growth Factor-independent Signaling Through the MAPK/Akt Pathway in Human Dermal Fibroblasts. Mol Cell Proteomics, 18(6):1123-1137(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: SWATH, redox signalling, skin fibroblasts, collagen, PDGF, MAPK |
Contact List
Adrian Paul Brown |
contact affiliation | Department of Biosciences, Durham University, UK |
contact email | a.p.brown@durham.ac.uk |
lab head | |
Adrian Brown |
contact affiliation | Durham University |
contact email | a.p.brown@durham.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD010747 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010747
- Label: PRIDE project
- Name: SWATH proteomics reveals that reductants selectively disrupt collagen homeostasis in human dermal fibroblasts and modify growth factor-independent signalling through the MAPK/Akt pathway