PXD010724

PXD010724 is an original dataset announced via ProteomeXchange.

Title	Nitric Oxide Enhances Resistance to PEG-induced Water Deficiency is Associated with the Primary Photosynthesis Reaction in Triticum aestivum L.
Description	Photosynthesis is affected by water deficiency (WD) stress, and nitric oxide (NO) is a free radical that participates in the photosynthesis process. Previous studies have suggested that NO regulates the excitation energy distribution of photosynthesis under WD stress. Here, quantitative phosphoproteomic profiling was conducted using isobaric tags for relative and absolute quantitation. Differentially phosphorylated protein species (DEPs) were identified in leaves of NO or polyethylene glycol (PEG)-treated wheat seedlings (D) and in control seedlings, 2,257 unique phosphorylated peptides and 2,416 phosphorylation sites were identified from 1,396 unique phosphoproteins. Of these, 96 DEPs displayed significant changes (≥ 1.50-fold, p < 0.01). These DEPs are involved in photosynthesis and signal transduction, etc. Furthermore, phosphorylation of several DEPs were up-regulated by both D and NO treatments, but down-regulated only in NO treatment. These differences affected the chlorophyll A-B binding protein, chloroplast post-illumination chlorophyll fluorescence increase protein, and SNT7, implying that NO indirectly regulated the absorption and transport of light energy in photosynthesis in response to WD stress. The significant difference of chlorophyll (Chl) content, Chl a fluorescence transient, photosynthesis index, and trapping and transport of light energy further indicated that exogenous NO under D stress enhanced the primary reaction of photosynthesis compared to D treatment. A putative pathway is proposed to elucidate NO regulation of the primary reaction of photosynthesis under WD.
HostingRepository	PRIDE
AnnounceDate	2019-11-14
AnnouncementXML	Submission_2019-11-14_02:17:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruixin Shao
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-08 06:50:46	ID requested
⏵ 1	2019-11-14 02:18:02	announced

Shao R, Zheng H, Jia S, Jiang Y, Yang Q, Kang G, L. Int J Mol Sci, 19(9):(2018) [pubmed]

curator keyword: Biological

submitter keyword: nitric oxide

20% PEG-induced water deficiency

phosphoproteomic

Triticum aestivum L.

primary reaction of photosynthesis

Shao Ruixin
contact affiliation	Collaborative Innovation Center of Henan Grain Crops and State Key Laboratory of Wheat and Maize Crop Science/College of Agronomy, Henan Agricultural University, Zhengzhou, China 450001
contact email	shao_rui_xin@126.com
lab head
Ruixin Shao
contact affiliation	Henan Agricultural University
contact email	shao_rui_xin@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010724
     1. Label: PRIDE project
     2. Name: Nitric Oxide Enhances Resistance to PEG-induced Water Deficiency is Associated with the Primary Photosynthesis Reaction in Triticum aestivum L.