PXD010697 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sleep-wake cycles drive daily dynamics of synaptic phosphorylation |
Description | The circadian clock drives daily changes of physiology, including sleep-wake cycles, by regulating transcription, protein abundance and function. Circadian phosphorylation controls cellular processes in peripheral organs, but little is known about its role in brain function and synaptic activity. We applied advanced quantitative phosphoproteomics to mouse forebrain synaptoneurosomes isolated across 24h, accurately quantifying almost 8,000 phosphopeptides. Remarkably, half of the synaptic phosphoproteins, including numerous kinases, had large-amplitude rhythms peaking at rest-activity and activity-rest transitions. Bioinformatic analyses revealed global temporal control of synaptic function via phosphorylation, including synaptic transmission, cytoskeleton reorganization and excitatory/inhibitory balance. Remarkably, sleep deprivation abolished 98% of all phosphorylation cycles in synaptoneurosomes, indicating that sleep-wake cycles rather than circadian signals are main drivers of synaptic phosphorylation, responding to both sleep and wake pressures. |
HostingRepository | PRIDE |
AnnounceDate | 2019-10-14 |
AnnouncementXML | Submission_2019-10-16_00:08:49.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mario Oroshi |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-06 06:15:40 | ID requested | |
1 | 2019-10-14 01:45:00 | announced | |
⏵ 2 | 2019-10-16 00:08:51 | announced | 2019-10-16: Updated publication reference for PubMed record(s): 31601740, 31601739. |
Publication List
Noya SB, Colameo D, Br, ü, ning F, Spinnler A, Mircsof D, Opitz L, Mann M, Tyagarajan SK, Robles MS, Brown SA, The forebrain synaptic transcriptome is organized by clocks but its proteome is driven by sleep. Science, 366(6462):(2019) [pubmed] |
Br, ü, ning F, Noya SB, Bange T, Koutsouli S, Rudolph JD, Tyagarajan SK, Cox J, Mann M, Brown SA, Robles MS, Sleep-wake cycles drive daily dynamics of synaptic phosphorylation. Science, 366(6462):(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: mass spectrometry, phosphoproteomics, phosphorylation, synaptoneurosome, circadian, sleep, EasyPhos |
Contact List
Maria S Robles |
contact affiliation | Institute of Medical Psychology, LMU Munich, Germany |
contact email | charo.robles@med.uni-muenchen.de |
lab head | |
Mario Oroshi |
contact affiliation | Proteomics |
contact email | oroshi@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010697
- Label: PRIDE project
- Name: Sleep-wake cycles drive daily dynamics of synaptic phosphorylation