PXD010694

PXD010694 is an original dataset announced via ProteomeXchange.

Title	β-actin RNA-BioID .
Description	Function and fate of mRNAs is controlled by RNA binding proteins (RBPs) but determining the proteome of a specific mRNA in vivo is still challenging. RNA proximity biotinylation on the transported β-actin mRNA tagged with MS2 aptamers (RNA-BioID) is used to characterize the dynamic proteome of the β-actin mRNP in mouse embryonic fibroblasts (MEFs). We have identified > 60 β-actin associated RBPs including all six previously known as well as novel interactors. By investigating the dynamics of the β-actin mRNP in MEFs, we expand the set of β-actin mRNA associated RBPs and characterize the changes of the interacting proteome upon serum-induced mRNA localization. We report that the KH-domain containing protein FUBP3 represents a new β-actin associated RBP that binds to its 3’-untranslated region outside the known RNA localization element but is required for β-actin RNA localization. RNA-BioID will allow to obtain a dynamic view on the composition of endogenous mRNPs.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:17:29.685.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mirita Franz-Wachtel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-06 05:35:29	ID requested
⏵ 1	2024-10-07 10:17:39	announced

Mukherjee J, Hermesh O, Eliscovich C, Nalpas N, Franz-Wachtel M, Ma, č, ek B, Jansen RP, -Actin mRNA interactome mapping by proximity biotinylation. Proc Natl Acad Sci U S A, 116(26):12863-12872(2019) [pubmed]

10.1073/pnas.1820737116;

curator keyword: Technical, Biological

submitter keyword: Mouse,RNA localization, RBPs, BioID

Ralf-Peter Jansen
contact affiliation	University of Tuebingen - Interfaculty Institute of Biochemistry
contact email	ralf.jansen@uni-tuebingen.de
lab head
Mirita Franz-Wachtel
contact affiliation	Proteome Center Tuebingen
contact email	mirita.franz@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD010694

PRIDE project URI

• PRIDE
  1. PXD010694
     1. Label: PRIDE project
     2. Name: β-actin RNA-BioID