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PXD010694

PXD010694 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titleβ-actin RNA-BioID .
DescriptionFunction and fate of mRNAs is controlled by RNA binding proteins (RBPs) but determining the proteome of a specific mRNA in vivo is still challenging. RNA proximity biotinylation on the transported β-actin mRNA tagged with MS2 aptamers (RNA-BioID) is used to characterize the dynamic proteome of the β-actin mRNP in mouse embryonic fibroblasts (MEFs). We have identified > 60 β-actin associated RBPs including all six previously known as well as novel interactors. By investigating the dynamics of the β-actin mRNP in MEFs, we expand the set of β-actin mRNA associated RBPs and characterize the changes of the interacting proteome upon serum-induced mRNA localization. We report that the KH-domain containing protein FUBP3 represents a new β-actin associated RBP that binds to its 3’-untranslated region outside the known RNA localization element but is required for β-actin RNA localization. RNA-BioID will allow to obtain a dynamic view on the composition of endogenous mRNPs.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:17:29.685.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMirita Franz-Wachtel
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-06 05:35:29ID requested
12024-10-07 10:17:39announced
Publication List
Mukherjee J, Hermesh O, Eliscovich C, Nalpas N, Franz-Wachtel M, Ma, č, ek B, Jansen RP, -Actin mRNA interactome mapping by proximity biotinylation. Proc Natl Acad Sci U S A, 116(26):12863-12872(2019) [pubmed]
10.1073/pnas.1820737116;
Keyword List
curator keyword: Technical, Biological
submitter keyword: Mouse,RNA localization, RBPs, BioID
Contact List
Ralf-Peter Jansen
contact affiliationUniversity of Tuebingen - Interfaculty Institute of Biochemistry
contact emailralf.jansen@uni-tuebingen.de
lab head
Mirita Franz-Wachtel
contact affiliationProteome Center Tuebingen
contact emailmirita.franz@uni-tuebingen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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