PXD010675

PXD010675 is an original dataset announced via ProteomeXchange.

Title	Peanut stunt virus–satellite RNA triggers changes in phosphorylation in N. benthamiana infected plants at the early stage of the infection
Description	Signaling in host plants is an integral part of a successful infection by pathogenic RNA viruses. Therefore, identifying early signaling events in host plants that play an important role in establishing the infection process will help our understanding of the disease process. In this context, phosphorylation constitutes one of the most important post-translational protein modifications, regulating many cellular processes. In this study, we aimed to identify the processes affected by peanut stunt virus (PSV) and its satellite RNA (satRNA) infection in Nicotiana benthamiana at the early stage of pathogenesis. To achieve this, we performed proteome and phosphoproteome analyses on plants treated with PSV and its satRNA. The analysis of the number of differentially phosphorylated proteins showed strong down-regulation in phosphorylation in virus-treated plants (without satRNA). Moreover, the proteome analysis revealed more down-regulated proteins in PSV+satRNA-treated plants, which indicated a complex dependence between proteins and their modifications. Apart from changes in photosynthesis and carbon metabolism usually observed in virus-infected plants, alterations in proteins involved in RNA synthesis, transport, and turnover were observed. This is the first community resource on the proteome and phosphoproteome of cucumovirus and its satRNA infection in N. benthamiana constituting a valuable data set for future studies.
HostingRepository	PRIDE
AnnounceDate	2018-10-25
AnnouncementXML	Submission_2018-10-25_08:49:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lam Dai Vu
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-03 03:00:59	ID requested
⏵ 1	2018-10-25 08:49:28	announced

Wrzesi, ń, ska B, Dai Vu L, Gevaert K, De Smet I, Obr, ę, palska-St, ę, plowska A, Infected Plants at the Early Stage of the Infection. Int J Mol Sci, 19(10):(2018) [pubmed]

curator keyword: Biological

submitter keyword: Signaling, virus infection, Nicotiana benthamiana, peanut stunt virus, plant-virus interactions, satellite RNA, phosphoproteome, RNA

Aleksandra Obrępalska-Stęplowska
contact affiliation	Institute of Plant Protection – National Research Institute, Department of Entomology, Animal Pests and Biotechnology, Władysława Węgorka 20, 60-318 Poznań, Poland
contact email	ao.steplowska@iorpib.poznan.pl
lab head
Lam Dai Vu
contact affiliation	VIB
contact email	lamvu@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010675

PRIDE project URI

• PRIDE
  1. PXD010675
     1. Label: PRIDE project
     2. Name: Peanut stunt virus–satellite RNA triggers changes in phosphorylation in N. benthamiana infected plants at the early stage of the infection