PXD010666

PXD010666 is an original dataset announced via ProteomeXchange.

Title	development of the cell wall in alfalfa
Description	In this study, the cell wall proteomes of 3 different regions of alfalfa stems were compared. These three regions correspond to three phases in the stem development. The sequential extraction of cell wall proteins with CaCl2, EGTA and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis. Although the highest similarities were observed between the apical and intermediate stem regions, the three proteome patterns are characteristic for each region. In the basal stem region, proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate. Beta-amylase and ferritin likewise accumulate more in the basal stem segment. Therefore remobilization of nutrients appears to be an important process in the oldest stem segment. The intermediate and the apical region are sites of cell wall polymer remodelling, as suggested by the high abundance of proteins involved in the remodelling of the cell wall such as xyloglucan endoglucosylase, polygalacturonase non-catalytic subunit or beta-galactosidase. The most striking change between the different stem parts is however the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem which suggests a particular role of this protein during the early development of stem tissues.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:40:26.168.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kjell sergeant
SpeciesList	scientific name: Medicago sativa (Alfalfa); NCBI TaxID: 3879;
ModificationList	dihydroxylated residue; (18)O label at both C-terminal oxygens; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	SCIEX TOF/TOF Series Explorer Software

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-02 08:01:09	ID requested
⏵ 1	2024-10-08 11:40:27	announced

10.3390/biology8030060;

Sergeant K, Printz B, Guerriero G, Renaut J, Lutts S, Hausman JF, The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems. Biology (Basel), 8(3):(2019) [pubmed]

curator keyword: Biological

submitter keyword: cell wall proteins, 2D-DIGE

Kjell Sergeant
contact affiliation	Luxembourg Institute of Science and Technology department: Environmental Research and Innnovation
contact email	kjell.sergeant@list.lu
lab head
kjell sergeant
contact affiliation	Luxembourg Institute of Science and Technology (LIST) Environmental Research and Innovation (ERIN)
contact email	kjell.sergeant@list.lu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD010666

PRIDE project URI

• PRIDE
  1. PXD010666
     1. Label: PRIDE project
     2. Name: development of the cell wall in alfalfa