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PXD010666

PXD010666 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titledevelopment of the cell wall in alfalfa
DescriptionIn this study, the cell wall proteomes of 3 different regions of alfalfa stems were compared. These three regions correspond to three phases in the stem development. The sequential extraction of cell wall proteins with CaCl2, EGTA and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis. Although the highest similarities were observed between the apical and intermediate stem regions, the three proteome patterns are characteristic for each region. In the basal stem region, proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate. Beta-amylase and ferritin likewise accumulate more in the basal stem segment. Therefore remobilization of nutrients appears to be an important process in the oldest stem segment. The intermediate and the apical region are sites of cell wall polymer remodelling, as suggested by the high abundance of proteins involved in the remodelling of the cell wall such as xyloglucan endoglucosylase, polygalacturonase non-catalytic subunit or beta-galactosidase. The most striking change between the different stem parts is however the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem which suggests a particular role of this protein during the early development of stem tissues.
HostingRepositoryPRIDE
AnnounceDate2024-10-08
AnnouncementXMLSubmission_2024-10-08_11:40:26.168.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterkjell sergeant
SpeciesList scientific name: Medicago sativa (Alfalfa); NCBI TaxID: 3879;
ModificationListdihydroxylated residue; (18)O label at both C-terminal oxygens; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentSCIEX TOF/TOF Series Explorer Software
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-02 08:01:09ID requested
12024-10-08 11:40:27announced
Publication List
10.3390/biology8030060;
Sergeant K, Printz B, Guerriero G, Renaut J, Lutts S, Hausman JF, The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems. Biology (Basel), 8(3):(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: cell wall proteins, 2D-DIGE
Contact List
Kjell Sergeant
contact affiliationLuxembourg Institute of Science and Technology department: Environmental Research and Innnovation
contact emailkjell.sergeant@list.lu
lab head
kjell sergeant
contact affiliationLuxembourg Institute of Science and Technology (LIST) Environmental Research and Innovation (ERIN)
contact emailkjell.sergeant@list.lu
dataset submitter
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Dataset FTP location
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