PXD010660

PXD010660 is an original dataset announced via ProteomeXchange.

Title	Nuclear versus cytoplasmic VHL interactomes
Description	Protein misfolding is linked to a wide variety of human disease. Protective cellular protein quality control (PQC) mechanisms evolved to selectively recognize misfolded proteins and limit their toxic effects. Using yeast as a model system, we previously discovered that the misfolded protein VHL interacts with various PQC machines on its way to being cleared by the ubiquitin-proteasome system. However, the clearance of nuclear and cytoplasmic VHL has difference PQC requirements. In this study, we performed SILAC-based AP-MS of nuclear (NLS) versus cytoplasmic (NES) VHL to identify, in an unbiased manner, the similarities and differences between nuclear and cytoplasmic PQC. We found that 4 molecular chaperones of the Hsp70 family, and multiple subunits of the proteasome, were significantly enriched with both nuclear and cytoplasmic VHL. By contrast, the chaperone Hsp90 and Sis1 were only associated with cytoplasmic VHL, whereas 6 of the 8 TRiC/chaperonin subunits were specifically enriched with nuclear VHL. Our study highlights unexpected differences between nuclear and cytoplasmic PQC, with important implications for our understanding of a wide range of protein misfolding diseases.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:47:33.492.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rahul Samant
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-02 01:17:40	ID requested
1	2018-11-04 15:58:04	announced
2	2018-11-21 06:11:52	announced	Updated publication reference for PubMed record(s): 30429547.
⏵ 3	2024-10-22 04:47:35	announced	2024-10-22: Updated project metadata.

Samant RS, Livingston CM, Sontag EM, Frydman J, Distinct proteostasis circuits cooperate in nuclear and cytoplasmic protein quality control. Nature, 563(7731):407-411(2018) [pubmed]

10.1038/s41586-018-0678-x;

curator keyword: Biological

submitter keyword: LC-MSMS, AP-MS,Yeast, SILAC, Orbitrap

Judith Frydman
contact affiliation	Department of Biology, Stanford University, Stanford, USA
contact email	jfrydman@stanford.edu
lab head
Rahul Samant
contact affiliation	Babraham Institute
contact email	rsamant@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010660

PRIDE project URI

• PRIDE
  1. PXD010660
     1. Label: PRIDE project
     2. Name: Nuclear versus cytoplasmic VHL interactomes