PXD010659

PXD010659 is an original dataset announced via ProteomeXchange.

Title	Changes in the Membrane-Associated Proteins of Exosomes Released from Human Macrophages after Mycobacterium tuberculosis Infection - Biotinylated
Description	Tuberculosis (TB) is the deadliest infectious disease worldwide. One obstacle hindering the elimination of TB is our lack of understanding of host-pathogen interactions. Exosomes, naturally loaded with microbial molecules, are circulating markers of TB. Changes in the host protein composition of exosomes from Mycobacterium tuberculosis (Mtb)-infected cells have not been described, can contribute to our understanding of the disease process, and serve as a direct source of biomarkers or as capture targets to enrich for exosomes containing microbial molecules. Here, the protein composition of exosomes from Mtb-infected and uninfected THP-1-derived macrophages was evaluated by tandem-mass-spectrometry and differences in protein abundances were assessed. Our results show that infection with Mtb leads to significant changes in the protein composition of exosomes. Specifically, 41 proteins were significantly more abundant in exosomes from Mtb-infected cells; 63% of these were predicted to be membrane associated. Thus, we used a novel biotinylation strategy to verify protein localization, and confirmed the localization of some of these proteins in the exosomal membrane. Our findings reveal another important scenario where Mtb could be influencing changes in host cells that unveil new features of the host-pathogen interaction and may also be exploited as a source of biomarkers for TB.
HostingRepository	PRIDE
AnnounceDate	2018-09-11
AnnouncementXML	Submission_2018-09-11_00:34:16.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010659
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nicole Kruh-Garcia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Sulfo-NHS-LC-LC-Biotin; Biotin; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-02 01:14:12	ID requested
1	2018-08-06 06:52:54	announced
⏵ 2	2018-09-11 00:34:17	announced	Updated project metadata.

Diaz G, Wolfe LM, Kruh-Garcia NA, Dobos KM, Changes in the Membrane-Associated Proteins of Exosomes Released from Human Macrophages after Mycobacterium tuberculosis Infection. Sci Rep, 6():37975(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: biotin, Asp-N

Karen M Dobos
contact affiliation	Department of Microbiology, Immunology and Pathology; Colorado State University, Fort Collins, CO
contact email	karen.dobos@colostate.edu
lab head
Nicole Kruh-Garcia
contact affiliation	Colorado State University
contact email	nicakruh@colostate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010659
     1. Label: PRIDE project
     2. Name: Changes in the Membrane-Associated Proteins of Exosomes Released from Human Macrophages after Mycobacterium tuberculosis Infection - Biotinylated