PXD010647

PXD010647 is an original dataset announced via ProteomeXchange.

Title	The Proteome of Enchytraeus crypticus – Exposure to CuO Nanomaterial and CuCl2
Description	The transcriptome of the ecotoxicological model Enchytraeus crypticus is well studied but the downstream changes at the protein level remained a gap. Changes in the protein regulation following exposure to CuO nanomaterial (NM) and Cu salt (CuCl2) were investigated. High performance liquid chromatography with tandem mass spectrometry using tandem mass tags was used. CuO NM elicited higher number of differentially expressed proteins compared to CuCl2 with little to no overlap of proteins. CuO NM caused more stress response mechanisms, with good agreement between differentially expressed proteins, genes and metabolites. CuCl2 caused higher impact in shorter time periods, but organisms have conserved mechanisms (constitutive genes) that allow Cu handling and detoxification. CuO NM caused higher impact after a longer exposure period, inducing regulation of facultative genes with a whole differentiated paradigm and cascade. This could be due to different issues: 1) the cell uptake route is different for Cu NM and Cu ions 2) internalized Cu NM can result in a “Trojan- horse” effect 3) the cascade of events occurs in a different time order 4) the organism uptake is different between life stages, i.e., cocoons thickened surface protects the entry of NM and juveniles have facilitated entry via tegument. Protein responses are of key importance when trying to understand the link between exposure and the related adverse biological effects. We here used advanced proteomic profiling techniques to describe how the protein responses differ when an organism is exposed to Copper (Cu) nanomaterial compared to when it is exposure to Cu ions. We observed that when organisms are exposed to the nano-form they have longer-term changes in protein patterns, patterns that are different from the responses following exposure to the ionic form. On the level of individual proteins we identified nano-specific mechanisms.
HostingRepository	PRIDE
AnnounceDate	2018-09-11
AnnouncementXML	Submission_2019-02-09_13:14:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Licha
SpeciesList	scientific name: Enchytraeus crypticus; NCBI TaxID: 913645;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-01 07:35:28	ID requested
1	2018-08-16 07:54:24	announced
2	2018-09-11 00:32:50	announced	Updated project metadata.
⏵ 3	2019-02-09 13:14:49	announced	Updated project metadata.

Maria VL, Licha D, Scott-Fordsmand JJ, Huber CG, Amorim MJB, -in Pursue of a Mechanistic Interpretation. Proteomics, 18(19):e1800091(2018) [pubmed]

curator keyword: Biological

submitter keyword: enchytraeids, metallic oxide nanomaterials, protein profiling, tandem mass tags

Christian Gottfried Huber
contact affiliation	Bioanalytical Research Labs, Department of Biology, University of Salzburg, Austria
contact email	c.huber@sbg.ac.at
lab head
David Licha
contact affiliation	University of Salzburg, Department of Biology, Bioanalytical Research Labs
contact email	david.licha@sbg.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010647

PRIDE project URI

• PRIDE
  1. PXD010647
     1. Label: PRIDE project
     2. Name: The Proteome of Enchytraeus crypticus – Exposure to CuO Nanomaterial and CuCl2