PXD010640

PXD010640 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of vocal fold fibroblasts exposed to cigarette smoke extract
Description	Reinke’s edema is a cigarette associated, benign, mostly bilateral lesion of the vocal folds leading to dysphonia and dyspnea. Until today information about pathophysiology is only scarce, treatment is only surgical. To explore the pathophysiology of Reinke’s edema, we exposed near primary human vocal fold fibroblasts to cigarette smoke extract enriched medium or air bubbled control for 24 hrs. followed by a proteomic analysis. Proteomic analyses revealed an increase of proteins involved in oxidative stress response. We furthermore found that a significant number of essential extracellular matrix proteins was altered by culturing human VFF with CSE enriched medium: UDP-glucose 6-dehydrogenase, a protein involved in hyaluronan synthesis was upregulated, while the expression of several fibrillar collagen types was significantly reduced. The current findings corroborate previous studies but revealed new insights in possible disease mechanisms of Reinke’s edema too. We postulate that changes in the composition -reduction of collagen fibrils, increase of hyaluronan- of the vocal folds’ ECM may lead to the clinical findings.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:48:05.137.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Darnhofer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-01 00:02:46	ID requested
1	2019-08-06 06:14:51	announced
⏵ 2	2024-10-22 04:48:07	announced	2024-10-22: Updated project metadata.

Gugatschka M, Darnhofer B, Grossmann T, Schittmayer M, Hortobagyi D, Kirsch A, Karpf E, Brcic L, Birner-Gruenberger R, Karbiener M, Proteomic Analysis of Vocal Fold Fibroblasts Exposed to Cigarette Smoke Extract: Exploring the Pathophysiology of Reinke's Edema. Mol Cell Proteomics, 18(8):1511-1525(2019) [pubmed]

10.1074/mcp.ra119.001272;

curator keyword: Biomedical

submitter keyword: cigarette smoke extract,fibroblasts, proteomics

Ruth Birner-Gruenberger
contact affiliation	Speaker of the Omics Center Head of Research Unit "Functional Proteomics and Metabolomic Pathways" Medical University Graz ZMF, Stiftingtalstrasse 24 8010 Graz Austria
contact email	ruth.birner-gruenberger@medunigraz.at
lab head
Barbara Darnhofer
contact affiliation	Omics Center Graz
contact email	barbara.darnhofer@klinikum-graz.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD010640

PRIDE project URI

• PRIDE
  1. PXD010640
     1. Label: PRIDE project
     2. Name: Proteomic analysis of vocal fold fibroblasts exposed to cigarette smoke extract