PXD010634 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Profiling N-myristoyltransferase activity and inhibition in breast cancer cells (MD-MB-231) using Sortase-A labelling |
Description | We report a novel application for Sortase A (SrtA), labeling N-terminal Gly proteins across the proteome at endogenous levels. We combined SrtA labeling of whole-cell lysates with a potent and selective NMT inhibitor, delivering a method capable of quantifying changes in cellular NMT activity in multiple substrates at the whole proteome level. This method shows good overlap in substrates with a previously reported YnMyr metabolic tagging strategy (PXD002687), and a similar capability to measure cellular NMT activity. The SrtA-based approach described here is complementary to YnMyr metabolic tagging, since the increase in signal for newly synthesized proteins exposing an N-terminal glycine mirrors the loss of signal seen with YnMyr. Importantly, this new method overcomes the limitations imposed by metabolic tagging. Since SrtA labeling is performed post-lysis this labeling strategy provides a readout of the cell status in the absence of any external perturbation and is applicable to virtually any type of biological sample, giving clear advantages over other in-cell labeling strategies. We anticipate that this method will enable the exploration of new avenues for NMT as a therapeutic target by providing a method for de novo identification of substrates most responsive to NMT inhibition, and a multifuctional target engagement biomarker assay suited to both animal models and patient biopsies. Besides shotgun proteomics, we coupled this robust NMT activity assay to a variety of other detection techniques, including gel-based analyses, and ELISA. This submission concerns myristoylated proteomes of human origin. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:47:31.656.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Remigiusz Serwa |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | myristoylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-31 07:39:28 | ID requested | |
1 | 2018-10-25 09:13:37 | announced | |
⏵ 2 | 2024-10-22 04:47:32 | announced | 2024-10-22: Updated project metadata. |
Publication List
Goya Grocin A, Serwa RA, Morales Sanfrutos J, Ritzefeld M, Tate EW, -Myristoyltransferase Activity and Inhibition Using Sortase A. Mol Cell Proteomics, 18(1):115-126(2019) [pubmed] |
10.1074/mcp.ra118.001043; |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Sortase A |
SrtA |
N-myristoylation |
NMT |
Contact List
Edward W Tate |
contact affiliation | Institute of Chemical Biology, Department of Chemistry, Imperial College London |
contact email | e.tate@imperial.ac.uk |
lab head | |
Remigiusz Serwa |
contact affiliation | Imperial College London |
contact email | r.serwa@imperial.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010634
- Label: PRIDE project
- Name: Profiling N-myristoyltransferase activity and inhibition in breast cancer cells (MD-MB-231) using Sortase-A labelling