PXD010629

PXD010629 is an original dataset announced via ProteomeXchange.

Title	Identification of CDC20/CDC20B partners in HEK cells demonstrates that CDC20, but not CDC20B, interacts with multiple APC/C components
Description	Multiciliated cells (MCCs) harbour dozens to hundreds of motile cilia, which beat in a synchronized and directional manner, thus generating hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation critically depends on the synthesis and release of numerous centrioles by specialized structures called deuterosomes. Little is known about the composition, organization and regulation of deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome-stage MCCs are characterized by the expression of many cell cycle-related genes. We further investigated the uncharacterized vertebrate specific cell division cycle 20B (CDC20B) gene, the host gene of microRNA-449abc. We show that the CDC20B protein associates to deuterosomes and is required for the release of centrioles and the subsequent production of cilia in mouse and Xenopus MCCs. CDC20B interacts with PLK1, which has been shown to coordinate centriole disengagement with the protease Separase in mitotic cells. Strikingly, over-expression of Separase rescued centriole disengagement and cilia production in CDC20B-deficient MCCs. This work reveals the shaping of a new biological function, deuterosome-mediated centriole production in vertebrate MCCs, by adaptation of canonical and recently evolved cell cycle-related molecules. A specific aim of this mass spectrometry experiment was to verify on immunoprecipitated protein complexes from CDC20 or CDC20B transfected HEK cells that CDC20, but not CDC20B, interacts with multiple APC/C components.
HostingRepository	PRIDE
AnnounceDate	2019-04-03
AnnouncementXML	Submission_2019-04-03_02:17:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pascal BARBRY
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-31 05:56:37	ID requested
⏵ 1	2019-04-03 02:17:25	announced

Revinski DR, Zaragosi LE, Boutin C, Ruiz-Garcia S, Deprez M, Thom, é V, Rosnet O, Gay AS, Mercey O, Paquet A, Pons N, Ponzio G, Marcet B, Kodjabachian L, Barbry P, CDC20B is required for deuterosome-mediated centriole production in multiciliated cells. Nat Commun, 9(1):4668(2018) [pubmed]

curator keyword: Biological

submitter keyword: HEK cells, centriole, deuterosome, motile cilia, CDC20B, Separase, PLK1, CDC20, cell cycle

Pascal Barbry
contact affiliation	Université Côte d'Azur, CNRS
contact email	barbry@ipmc.cnrs.fr
lab head
Pascal BARBRY
contact affiliation	Université Côte d'Azur
contact email	barbry@ipmc.cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010629
     1. Label: PRIDE project
     2. Name: Identification of CDC20/CDC20B partners in HEK cells demonstrates that CDC20, but not CDC20B, interacts with multiple APC/C components