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PXD010629

PXD010629 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of CDC20/CDC20B partners in HEK cells demonstrates that CDC20, but not CDC20B, interacts with multiple APC/C components
DescriptionMulticiliated cells (MCCs) harbour dozens to hundreds of motile cilia, which beat in a synchronized and directional manner, thus generating hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation critically depends on the synthesis and release of numerous centrioles by specialized structures called deuterosomes. Little is known about the composition, organization and regulation of deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome-stage MCCs are characterized by the expression of many cell cycle-related genes. We further investigated the uncharacterized vertebrate specific cell division cycle 20B (CDC20B) gene, the host gene of microRNA-449abc. We show that the CDC20B protein associates to deuterosomes and is required for the release of centrioles and the subsequent production of cilia in mouse and Xenopus MCCs. CDC20B interacts with PLK1, which has been shown to coordinate centriole disengagement with the protease Separase in mitotic cells. Strikingly, over-expression of Separase rescued centriole disengagement and cilia production in CDC20B-deficient MCCs. This work reveals the shaping of a new biological function, deuterosome-mediated centriole production in vertebrate MCCs, by adaptation of canonical and recently evolved cell cycle-related molecules. A specific aim of this mass spectrometry experiment was to verify on immunoprecipitated protein complexes from CDC20 or CDC20B transfected HEK cells that CDC20, but not CDC20B, interacts with multiple APC/C components.
HostingRepositoryPRIDE
AnnounceDate2019-04-03
AnnouncementXMLSubmission_2019-04-03_02:17:24.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPascal BARBRY
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-31 05:56:37ID requested
12019-04-03 02:17:25announced
Publication List
Revinski DR, Zaragosi LE, Boutin C, Ruiz-Garcia S, Deprez M, Thomé V, Rosnet O, Gay AS, Mercey O, Paquet A, Pons N, Ponzio G, Marcet B, Kodjabachian L, Barbry P, CDC20B is required for deuterosome-mediated centriole production in multiciliated cells. Nat Commun, 9(1):4668(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: HEK cells, centriole, deuterosome, motile cilia, CDC20B, Separase, PLK1, CDC20, cell cycle
Contact List
Pascal Barbry
contact affiliationUniversité Côte d'Azur, CNRS
contact emailbarbry@ipmc.cnrs.fr
lab head
Pascal BARBRY
contact affiliationUniversité Côte d'Azur
contact emailbarbry@ipmc.cnrs.fr
dataset submitter
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Dataset FTP location
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