PXD010624

PXD010624 is an original dataset announced via ProteomeXchange.

Title	Heterotrophic carbon metabolism and energy acquisition in Candidatus Thioglobus singularis strain PS1, a member of the SUP05 clade of marine Gammaproteobacteria
Description	The balance between autotrophy and heterotrophy regulates the size of the ocean’s carbon sink. The contribution of a particular bacterial or archaeal lineage to carbon flux is determined by its metabolism, which may vary within lineages and may depend on environmental conditions. Members of the SUP05 clade are often described as a single lineage of chemoautotrophic gammaproteobacterial sulfur-oxidizers (GSOs). Like other SUP05, the Arctic96BD-19 subclade contains the genetic potential for carbon fixation through the Calvin cycle yet differs from other strictly chemoautotrophic SUP05 in its potential to also consume exogenous organic matter from surrounding seawater. The balance between organic carbon production and consumption in Arctic96BD-19 is not well understood and could have global implications to carbon cycling in the world’s oceans. Here we used genomic reconstructions, physiological growth experiments, and proteomics to characterize central carbon and energy processing of Ca. Thioglobus singularis strain PS1, a representative of the Arctic96BD-19 subclade of SUP05 that was isolated from Puget Sound, WA, USA. The addition of either complex organic matter from phytoplankton lysate or individual phytoplankton-derived organic compounds significantly enhanced the growth of PS1. Proteins involved in methylotrophic pathways for carbon assimilation and energy generation were significantly upregulated when lysate was added to the growth media, suggesting that PS1 uses methylated compounds derived from marine organisms. Although sulfur oxidation and carbon fixation are defining characteristics of the GSO clade, strain PS1 did not require reduced inorganic sulfur for growth and there was little evidence of carbon fixation. These data indicate that strain PS1 functions primarily as an organic carbon consumer, suggesting that the role of widespread Arctic96BD-19 may be largely heterotrophic.
HostingRepository	PRIDE
AnnounceDate	2019-04-12
AnnouncementXML	Submission_2019-04-12_01:14:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rachel Lundeen
SpeciesList	scientific name: Candidatus Thioglobus singularis PS1; NCBI TaxID: 1125411;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-31 01:52:29	ID requested
⏵ 1	2019-04-12 01:15:00	announced

Spietz RL, Lundeen RA, Zhao X, Nicastro D, Ingalls AE, Morris RM, Heterotrophic carbon metabolism and energy acquisition in Candidatus Thioglobus singularis strain PS1, a member of the SUP05 clade of marine Gammaproteobacteria. Environ Microbiol, 21(7):2391-2401(2019) [pubmed]

curator keyword: Biological

submitter keyword: Marine Gammaproteobacteria, SUP05, Arctic96BD-19, Candidatus Thioglobus singularis strain PS1

Anitra Ingalls
contact affiliation	University of Washington
contact email	aingalls@uw.edu
lab head
Rachel Lundeen
contact affiliation	University of Washington
contact email	ralundeen@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010624
     1. Label: PRIDE project
     2. Name: Heterotrophic carbon metabolism and energy acquisition in Candidatus Thioglobus singularis strain PS1, a member of the SUP05 clade of marine Gammaproteobacteria