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PXD010622

PXD010622 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleToxoplasma gondii MIC2 TSRs glycosylation
DescriptionToxoplasma gondii is an intracellular parasite that causes disseminated infections which can lead to neurological damage in fetuses and immunocompromised individuals. Microneme protein 2 (MIC2), a member of the thrombospondin-related anonymous protein (TRAP) family, is a secreted protein important for motility, host cell attachment, invasion, and egress. MIC2 contains six thrombospondin type I repeats (TSRs) that are modified by C-mannose and O-fucose in Plasmodium spp. and mammals. Here we used mass spectrometry to show that the four TSRs in T. gondii MIC2 with protein O-fucosyltransferase 2 (POFUT2) acceptor sites are modified by a dHexHex disaccharide, while Trp residues within three TSRs are also modified with C-mannose. Disruption of genes encoding either pofut2 or nucleotide sugar transporter 2 (nst2), the putative GDP-fucose transporter, results in loss of MIC2 O-fucosylation, as detected by an antibody against the GlcFuc disaccharide, and markedly reduced cellular levels of MIC2. Furthermore, in 10-15% of the Δpofut2 or Δnst2 vacuoles, MIC2 accumulates earlier in the secretory pathway rather than localizing to micronemes. Dissemination of tachyzoites in human foreskin fibroblasts is reduced in these knockouts, which both show defects in attachment to and invasion of host cells comparable to the phenotype observed in the Δmic2. These results, which show O-fucosylation of TSRs is required for efficient processing of MIC2 and for normal parasite invasion, are consistent with the recent demonstration that P. falciparum Δpofut2 has decreased virulence and support a conserved role for this glycosylation pathway in quality control of TSR-containing proteins in eukaryotes.
HostingRepositoryPRIDE
AnnounceDate2018-09-11
AnnouncementXMLSubmission_2018-09-11_00:34:12.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGiulia Bandini
SpeciesList scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deoxyhexosylated; iodoacetamide derivatized residue; hexosylated residue
InstrumentQ Exactive; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-31 01:46:36ID requested
12018-08-09 09:49:53announced
22018-09-11 00:34:12announcedUpdated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: O-fucosylation, C-mannosylation, Toxoplasma gondii
Contact List
Catherine Elizabeth Costello
contact affiliationDepartment of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, MA 02118, USA
contact emailcecmsms@bu.edu
lab head
Giulia Bandini
contact affiliationBoston University
contact emailgbandini@bu.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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