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PXD010621 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMulti-Omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency
DescriptionPluripotency is highly dynamic and progresses through a continuum of pluripotent stem-cell states. The two states that bookend the pluripotency continuum, na??ve and primed, are well characterized, but our understanding of the intermediate states and transitions between them remain incomplete. Here, we dissect the dynamics of pluripotent state transitions underlying pre- to post-implantation epiblast differentiation. Through comprehensive mapping of the proteome, phosphoproteome, transcriptome, and epigenome of embryonic stem cells transitioning from na??ve to primed pluripotency, we find that rapid, acute, and widespread changes to the phosphoproteome precede ordered changes to the epigenome, transcriptome, and proteome. Reconstruction of kinase-substrate networks reveals signaling cascades, dynamics, and crosstalk. Distinct waves of global proteomic changes mark discrete phases of pluripotency, with cell state-specific surface markers tracking pluripotent state transitions. Our data provide new insights into the multi-layered control of the phased progression of pluripotency and a foundation for modeling mechanisms regulating pluripotent state transitions (www.stemcellatlasorg).
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMario Oroshi
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-30 07:53:00ID requested
12019-05-07 08:38:35announced
22019-05-10 02:53:49announcedUpdated project metadata.
32019-06-03 01:31:07announcedUpdated project metadata.
Publication List
Yang P, Humphrey SJ, Cinghu S, Pathania R, Oldfield AJ, Kumar D, Perera D, Yang JYH, James DE, Mann M, Jothi R, Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency. Cell Syst, 8(5):427-445.e10(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: mass spectrometry, proteomics, phosphoproteomics, phosphorylation, signal transduction, post-translational modification, EasyPhos, stem cell, differentiation, mESC, EpiLC, embryonic, pluripotency, dynamic
Contact List
Matthias Mann
contact affiliationDepartment of Proteomics and Signal Transduction Max Planck Institute of Biochemistry
contact emailmmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliationProteomics
contact emailoroshi@biochem.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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