PXD010609 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Posttranslational modifications regulate cellular responses upon UV-induced DNA damage |
Description | SUMOylation is a posttranslational protein modification which is characterized by the covalent attachment of a small 11kDa protein, called Small Ubiquitin-like MOdifier (SUMO). SUMOylation plays a pivotal role in a multitude of cellular pathways including cellular responses upon DNA damage. Here, we identified multiple proteins which are SUMOylated in U2OS cells in response to ultraviolet light (UV) irradiation and ionizing radiation (IR). We show that the SUMOylation response upon UV irradiation was more pronounced compared to the response upon IR. The major SUMOylation target upon UV-irradiation was the transcription-coupled nucleotide excision repair (TC-NER) protein, Cockayne Syndrome B (CSB). This protein plays an important role in the repair of UV-induced lesions in actively transcribed genes. In a second proteomic approach we identified SUMOylation-dependent and independent protein interactors of the N-terminus of CSB. Here, we uncovered that the affinity of multiple RNA polymerase-associated proteins towards CSB is influenced by SUMOylation. Finally, we set out to identify ubiquitination events upon UV-irradiation which are influenced by the CSA-ubiquitin ligase complex, which is also involved in TC-NER and is closely connected to CSB, because mutations in either CSA or CSB result in the same phenotype, Cockayne syndrome. We found that RPB1, the major subunit of RNA polymerase II, was ubiquitinated in a CSA-dependent manner upon UV which finally led to its degradation. |
HostingRepository | PRIDE |
AnnounceDate | 2019-10-11 |
AnnouncementXML | Submission_2019-11-19_16:13:37.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Frauke Liebelt |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-30 02:10:34 | ID requested | |
1 | 2019-10-11 12:01:12 | announced | |
⏵ 2 | 2019-11-19 16:13:38 | announced | 2019-11-20: Updated publication reference for PubMed record(s): 31722399. |
Publication List
Liebelt F, Schimmel J, Verlaan-de Vries M, Klemann E, van Royen ME, van der Weegen Y, Luijsterburg MS, Mullenders LH, Pines A, Vermeulen W, Vertegaal ACO, Transcription-coupled nucleotide excision repair is coordinated by ubiquitin and SUMO in response to ultraviolet irradiation. Nucleic Acids Res, 48(1):231-248(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Human, DNA damage, SUMOylation, post-translational modification, CSB, CSA |
Contact List
Alfred Vertegaal |
contact affiliation | Leiden University Medical Center (LUMC) Einthovenweg 20 2333 ZC Leiden |
contact email | a.c.o.vertegaal@lumc.nl |
lab head | |
Frauke Liebelt |
contact affiliation | Leiden University Medical Centre |
contact email | F.Liebelt@lumc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010609
- Label: PRIDE project
- Name: Posttranslational modifications regulate cellular responses upon UV-induced DNA damage