PXD010590

PXD010590 is an original dataset announced via ProteomeXchange.

Title	Proteomics Analysis of Escherichia coli Persisters
Description	Persisters, a dormant and multi-drug tolerant subpopulation that are able to resuscitate after antibiotic treatment, have recently received considerable attentions as the major risk of the relapse of various infectious diseases in clinics. However, due to their low abundance and inherent mutability, it is extremely difficult to study them by proteomics. Here, we developed a magnetic beads-based separation approach to enrich Escherichia coli persisters and then subject them to Filter-Aided Sample Perparation (FASP) followed by LC-MS/MS analysis. We applied spectral counting-based quantitative proteomics to study the proteomic changes of E. coli persisters under high concentration of ampicillin treatment.
HostingRepository	PRIDE
AnnounceDate	2018-10-16
AnnouncementXML	Submission_2018-10-26_03:07:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jordy Evan Sulaiman
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-27 02:13:55	ID requested
1	2018-10-16 07:23:56	announced
⏵ 2	2018-10-26 03:07:42	announced	Updated publication reference for PubMed record(s): 30336045.

Sulaiman JE, Hao C, Lam H, Specific Enrichment and Proteomics Analysis of Escherichia coli Persisters from Rifampin Pretreatment. J Proteome Res, 17(11):3984-3996(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: dormancy, tolerant, persistence, antibiotic, proteomics

Henry Lam
contact affiliation	Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST), Hong Kong
contact email	kehlam@ust.hk
lab head
Jordy Evan Sulaiman
contact affiliation	The Hong Kong University of Science and Technology (HKUST)
contact email	jesulaiman@connect.ust.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010590

PRIDE project URI

• PRIDE
  1. PXD010590
     1. Label: PRIDE project
     2. Name: Proteomics Analysis of Escherichia coli Persisters