PXD010585

PXD010585 is an original dataset announced via ProteomeXchange.

Title	A large-scale proteomic identification of targets of cellular miR-197 downregulated by enterovirus A71
Description	MicroRNAs are noncoding RNA species comprising 18–23 nucleotides that regulate host-virus interaction networks. Here, we showed that enterovirus A71 infection in human rhabdomyosarcoma (RD) involved miR-197 expression. miR-197 can regulate virus replication in the context of viral RNA synthesis via the transfection of its mimic into RD cells. We employed a mass spectrometry-based quantitative proteomic stable isotope labeling with amino acids in cell culture (SILAC) approach for the identification of miR-197 target genes by transfecting mimetic miR-197 into RD cells, and the differential expression of prospective target proteins was identified. A total of 1,822 genes were repeatedly and considerably downregulated in miR-197-transfected RD cells, 106 of which were predicted to have seed sites by TargetScan. Seven of the selected 8 genes potentially related to viral replication and immune response were confidently validated as direct miR-197 targets using a luciferase (untranslated region (3'-UTR)) reporter assay. The expression of three selected endogenous molecules (ITGAV, ETF1, and MAP2K1 (MEK1)) was significantly reduced when RD cells were transfected with an miR-197 mimic. Our results provide a database of miR-197 targets, which is potentially of interest in the area of viral pathogenesis and other research fields.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:25.160.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WEN-FANG TANG
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-27 01:24:10	ID requested
1	2018-10-24 07:44:27	announced
⏵ 2	2024-10-22 04:36:33	announced	2024-10-22: Updated project metadata.

Tang WF, Huang RT, Chien KY, Tang P, Horng JT, Large-Scale Proteomic Identification of Targets of Cellular miR-197 Downregulated by Enterovirus A71. J Proteome Res, 18(1):449-460(2019) [pubmed]

10.1021/acs.jproteome.8b00762;

curator keyword: Biological

submitter keyword: viral replication, TargetScan, microRNA, SILAC, miR-197,enterovirus A71

Jim-Tong Horng
contact affiliation	Department of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, No.259, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33302, Taiwan (R.O.C.)
contact email	jimtong@mail.cgu.edu.tw
lab head
WEN-FANG TANG
contact affiliation	CGU
contact email	cico6815@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010585

PRIDE project URI

• PRIDE
  1. PXD010585
     1. Label: PRIDE project
     2. Name: A large-scale proteomic identification of targets of cellular miR-197 downregulated by enterovirus A71