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PXD010585

PXD010585 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA large-scale proteomic identification of targets of cellular miR-197 downregulated by enterovirus A71
DescriptionMicroRNAs are noncoding RNA species comprising 18–23 nucleotides that regulate host-virus interaction networks. Here, we showed that enterovirus A71 infection in human rhabdomyosarcoma (RD) involved miR-197 expression. miR-197 can regulate virus replication in the context of viral RNA synthesis via the transfection of its mimic into RD cells. We employed a mass spectrometry-based quantitative proteomic stable isotope labeling with amino acids in cell culture (SILAC) approach for the identification of miR-197 target genes by transfecting mimetic miR-197 into RD cells, and the differential expression of prospective target proteins was identified. A total of 1,822 genes were repeatedly and considerably downregulated in miR-197-transfected RD cells, 106 of which were predicted to have seed sites by TargetScan. Seven of the selected 8 genes potentially related to viral replication and immune response were confidently validated as direct miR-197 targets using a luciferase (untranslated region (3'-UTR)) reporter assay. The expression of three selected endogenous molecules (ITGAV, ETF1, and MAP2K1 (MEK1)) was significantly reduced when RD cells were transfected with an miR-197 mimic. Our results provide a database of miR-197 targets, which is potentially of interest in the area of viral pathogenesis and other research fields.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:36:25.160.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWEN-FANG TANG
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-27 01:24:10ID requested
12018-10-24 07:44:27announced
22024-10-22 04:36:33announced2024-10-22: Updated project metadata.
Publication List
Tang WF, Huang RT, Chien KY, Tang P, Horng JT, Large-Scale Proteomic Identification of Targets of Cellular miR-197 Downregulated by Enterovirus A71. J Proteome Res, 18(1):449-460(2019) [pubmed]
10.1021/acs.jproteome.8b00762;
Keyword List
curator keyword: Biological
submitter keyword: viral replication, TargetScan, microRNA, SILAC, miR-197,enterovirus A71
Contact List
Jim-Tong Horng
contact affiliationDepartment of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, No.259, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33302, Taiwan (R.O.C.)
contact emailjimtong@mail.cgu.edu.tw
lab head
WEN-FANG TANG
contact affiliationCGU
contact emailcico6815@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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