PXD010585 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A large-scale proteomic identification of targets of cellular miR-197 downregulated by enterovirus A71 |
Description | MicroRNAs are noncoding RNA species comprising 18–23 nucleotides that regulate host-virus interaction networks. Here, we showed that enterovirus A71 infection in human rhabdomyosarcoma (RD) involved miR-197 expression. miR-197 can regulate virus replication in the context of viral RNA synthesis via the transfection of its mimic into RD cells. We employed a mass spectrometry-based quantitative proteomic stable isotope labeling with amino acids in cell culture (SILAC) approach for the identification of miR-197 target genes by transfecting mimetic miR-197 into RD cells, and the differential expression of prospective target proteins was identified. A total of 1,822 genes were repeatedly and considerably downregulated in miR-197-transfected RD cells, 106 of which were predicted to have seed sites by TargetScan. Seven of the selected 8 genes potentially related to viral replication and immune response were confidently validated as direct miR-197 targets using a luciferase (untranslated region (3'-UTR)) reporter assay. The expression of three selected endogenous molecules (ITGAV, ETF1, and MAP2K1 (MEK1)) was significantly reduced when RD cells were transfected with an miR-197 mimic. Our results provide a database of miR-197 targets, which is potentially of interest in the area of viral pathogenesis and other research fields. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:36:25.160.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | WEN-FANG TANG |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-27 01:24:10 | ID requested | |
1 | 2018-10-24 07:44:27 | announced | |
⏵ 2 | 2024-10-22 04:36:33 | announced | 2024-10-22: Updated project metadata. |
Publication List
Tang WF, Huang RT, Chien KY, Tang P, Horng JT, Large-Scale Proteomic Identification of Targets of Cellular miR-197 Downregulated by Enterovirus A71. J Proteome Res, 18(1):449-460(2019) [pubmed] |
10.1021/acs.jproteome.8b00762; |
Keyword List
curator keyword: Biological |
submitter keyword: viral replication, TargetScan, microRNA, SILAC, miR-197,enterovirus A71 |
Contact List
Jim-Tong Horng |
contact affiliation | Department of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, No.259, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33302, Taiwan (R.O.C.) |
contact email | jimtong@mail.cgu.edu.tw |
lab head | |
WEN-FANG TANG |
contact affiliation | CGU |
contact email | cico6815@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010585
- Label: PRIDE project
- Name: A large-scale proteomic identification of targets of cellular miR-197 downregulated by enterovirus A71