PXD010584

PXD010584 is an original dataset announced via ProteomeXchange.

Title	PID14 processing by Maize AF PLCPs
Description	Ustilago maydis is a biotrophic fungus causing corn smut disease in maize. To downregulate immune responses and promote host colonization, U. maydis secretes a set of effector proteins into the plant apoplast. An effector essential for U. maydis virulence is Pit2, an inhibitor of papain-like cysteine proteases (PLCPs). Pit2 virulence function relies on a 14 amino acids motif (PID14). While sequence of the Pit2 effector is highly diverse amongst related pathogen species, the PID14 motif is highly conserved. Interestingly, synthetic PID14 peptides act more efficiently as PLCP inhibitors than the full-length Pit2 effector. In line with this finding, mass spectrometry showed processing of Pit2 by maize PLCPs, which releases an inhibitory core motif of the PID14. Mutational analysis demonstrated that two residues of the released inhibitor peptide are essential for Pit2 function and, consequently, for U. maydis virulence. Based on these findings, we propose a model, in which the Pit2 effector functions as a decoy: Pit2 represents a favorable substrate for apoplastic PLCPs, which are central hubs of the maize immune system. Processing of Pit2 releases the inhibitor peptide, which in turn efficiently blocks PLCPs to modulate host immunity.
HostingRepository	PRIDE
AnnounceDate	2019-02-25
AnnouncementXML	Submission_2019-04-12_01:03:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fatih Demir
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	acetylated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-27 00:59:49	ID requested
1	2019-02-25 06:22:23	announced
⏵ 2	2019-04-12 01:04:00	announced	Updated publication reference for PubMed record(s): 30952847.

Misas Villamil JC, Mueller AN, Demir F, Meyer U, Ö, kmen B, Schulze H, ü, ynck J, Breuer M, Dauben H, Win J, Huesgen PF, Doehlemann G, A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif. Nat Commun, 10(1):1576(2019) [pubmed]

curator keyword: Biological

submitter keyword: PID14 Maize AF PLCPs Apoplastic Fluid

Pitter Florian Huesgen
contact affiliation	Forschungszentrum Juelich ZEA-3 Analytics Wilhelm-Johnen-Str D-52425 Juelich Germany
contact email	p.huesgen@fz-juelich.de
lab head
Fatih Demir
contact affiliation	Forschungszentrum Juelich ZEA-3 Analytics Wilhelm-Johnen-Str D-52425 Juelich
contact email	f.demir@fz-juelich.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD010584

PRIDE project URI

• PRIDE
  1. PXD010584
     1. Label: PRIDE project
     2. Name: PID14 processing by Maize AF PLCPs