PXD010579

PXD010579 is an original dataset announced via ProteomeXchange.

Title	Stable-protein Pair Analysis as A Novel Strategy to Identify Proteomic Signatures: Application to Seminal Plasma From Infertile Patients.
Description	Our aim was to define seminal plasma proteome signatures of infertile patients categorized according to their seminal parameters using TMT-LC-MS/MS. To that extent, quantitative proteomic data was analyzed following two complementary strategies: (i) the conventional approach based on standard statistical analyses of relative protein quantification values; and (ii) a novel strategy focused on establishing stable-protein pairs. By conventional analyses, the abundance of some seminal plasma proteins was found to be positively correlated with sperm concentration. However, this correlation was not found for all the peptides within a specific protein, bringing to light the high heterogeneity existing in the seminal plasma proteome due to both the proteolytic fragments and/or the post-translational modifications. This issue was overcome by conducting the novel stable-protein pairs analysis proposed herein. A total of 182 correlations comprising 24 different proteins were identified in the normozoospermic-control population, while this proportion was drastically reduced in infertile patients with altered seminal parameters (18 in patients with reduced sperm motility, 0 in patients with low sperm concentration and 3 in patients with no sperm in the ejaculate). These results suggest the existence of multiple etiologies causing the same alteration in seminal parameters. Additionally, the repetition of the stable-protein pair analysis in the control group by adding the data from a single patient at a time enabled to identify alterations in the stable-protein pairs profile of individual patients with altered seminal parameters. These results suggest potential underlying pathogenic mechanisms in individual infertile patients, and might open up a window to its application in the personalized diagnostic of male infertility.
HostingRepository	PRIDE
AnnounceDate	2019-02-25
AnnouncementXML	Submission_2019-03-21_04:52:57.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010579
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rafael Oliva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-26 08:42:25	ID requested
1	2019-02-24 23:57:02	announced
⏵ 2	2019-03-21 04:52:58	announced	Updated publication reference for PubMed record(s): 30518674.

Barrachina F, Jodar M, Delgado-Due, ñ, as D, Soler-Ventura A, Estanyol JM, Mallofr, é C, Ballesc, à JL, Oliva R, Stable-protein Pair Analysis as A Novel Strategy to Identify Proteomic Signatures: Application To Seminal Plasma From Infertile Patients. Mol Cell Proteomics, 18(Suppl 1):S77-S90(2019) [pubmed]

curator keyword: Biological

submitter keyword: Male infertility, seminal plasma, proteomics, TMT, stable-protein pairs

Rafael Oliva Virgili
contact affiliation	Genetics Unit, Department of Biomedical Sciences, Faculty of Medicine, Casanova 143, 08036 Barcelona, Spain
contact email	roliva@ub.edu
lab head
Rafael Oliva
contact affiliation	Director Molecular Biology of Reproduction and Development research Group, Genetics Unit, Faculty of Medicine, University of Barcelona, Institut d'Investigacions Biomèdiques August Pi iSunyer (IDIBAPS), and Hospital Clinic, Barcelona, Spain.
contact email	roliva@ub.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD010579

PRIDE project URI

• PRIDE
  1. PXD010579
     1. Label: PRIDE project
     2. Name: Stable-protein Pair Analysis as A Novel Strategy to Identify Proteomic Signatures: Application to Seminal Plasma From Infertile Patients.