PXD010563

PXD010563 is an original dataset announced via ProteomeXchange.

Title	UoL_turnover_SRM (Holman et al. 2016)
Description	Protein turnover represents an important mechanism in the functioning of cells, with deregulated synthesis and degradation of proteins implicated in many diseased states. Therefore, proteomics strategies to measure, with high confidence, turnover rates are of vital importance to understanding many biological processes. In this study, the more widely used approach of non-targeted precursor ion signal intensity (MS1) quantification is compared to selected reaction monitoring (SRM), a data acquisition strategy that records data for specific peptides, to determine if improved quantitative data would be obtained using a targeted quantification approach. Using mouse liver as a model system, turnover measurement of four tricarboxylic acid cycle proteins was performed using both MS1 and SRM quantification strategies. SRM outperformed MS1 in terms of sensitivity and selectivity of measurement, allowing more confident determination of protein turnover rates. SRM data is acquired using cheaper and more widely available tandem quadrupole mass spectrometers, making the approach accessible to a larger number of researchers than MS1 quantification, which is best performed on high mass resolution instruments. SRM acquisition is ideally suited to focussed studies where the turnover of tens of proteins is measured, making it applicable in determining the dynamics of proteins complexes and complete metabolic pathways.
HostingRepository	PeptideAtlas
AnnounceDate	2018-07-25
AnnouncementXML	Submission_2018-07-25_10:11:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stephen Holman
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; 6x(13)C labeled L-lysine
Instrument	TSQ Vantage

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-25 10:08:47	ID requested
⏵ 1	2018-07-25 10:11:17	announced

Holman SW, Hammond DE, Simpson DM, Waters J, Hurst JL, Beynon RJ, Protein turnover measurement using selected reaction monitoring-mass spectrometry (SRM-MS). Philos Trans A Math Phys Eng Sci, 374(2079):(2016) [pubmed]

curator keyword: selected reaction monitoring, SRM, targeted

Stephen Holman
contact affiliation	University of Liverpool
contact email	stephen.holman@liverpool.ac.uk
dataset submitter
Professor Robert J. Beynon
contact affiliation	University of Liverpool
contact email	r.beynon@liv.ac.uk
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI