PXD010559

PXD010559 is an original dataset announced via ProteomeXchange.

Title	Proteome of the rodent malaria parasite Plasmodium berghei liver stage merosomes
Description	We use the Plasmodium berghei rodent model to characterize the proteome of the final phase of liver stage development, the merosomes, packets of hepatic merozoites that bud from the host hepatocyte to initiate the blood stage of malaria. Plasmodium berghei WT ANKA strain was used to infect HepG2 hepatoma cells. Samples were fractionated by strong cation exchange, and nano-LC Orbitrap mass spectrometry was used to perform untargeted proteomic profiling of 3 biological replicates. Data was processed using MaxQuant and LFQ. Additional searches were performed to identify peptides from cleaved acetylated PEXELs (protein export elements) to identify proteins putatively exported to the host hepatocyte during liver stage development.
HostingRepository	PRIDE
AnnounceDate	2019-12-11
AnnouncementXML	Submission_2019-12-11_07:36:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823; scientific name: Anopheles stephensi (Asian malaria mosquito); NCBI TaxID: 30069; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-25 08:09:05	ID requested
1	2019-03-25 03:44:37	announced
⏵ 2	2019-12-11 07:36:40	announced	2019-12-11: Updated publication reference for PubMed record(s): 31335145.

Shears MJ, Sekhar Nirujogi R, Swearingen KE, Renuse S, Mishra S, Jaipal Reddy P, Moritz RL, Pandey A, Sinnis P, Merosomes: The Link between Liver and Blood Stages in Malaria. J Proteome Res, 18(9):3404-3418(2019) [pubmed]

ProteomeXchange project tag: Liver (B/D-HPP), Malaria Host-Pathogen Interaction Center (MaHPIC)

curator keyword: Biological

submitter keyword: Plasmodium, merosomes, malaria, proteomics

Akhilesh Pandey
contact affiliation	733 N. Broadway Miller Research Building room 527 Johns Hopkins University Baltimore, MD 21205
contact email	pandey@jhmi.edu
lab head
Akhilesh Pandey
contact affiliation	Johns Hopkins University
contact email	pandey@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD010559

PRIDE project URI

• PRIDE
  1. PXD010559
     1. Label: PRIDE project
     2. Name: Proteome of the rodent malaria parasite Plasmodium berghei liver stage merosomes