PXD010554
PXD010554 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | System-wide identification of enzyme substrates by thermal analysis (SIESTA) |
Description | Despite the immense importance of enzyme-substrate reactions, there is a lack of general and unbiased tools for identifying the molecular components participating in these reactions on a cellular level. Here we developed a universal method called System-wide Identification of Enzyme Substrates by Thermal Analysis (SIESTA). The approach assumes that post-translational modification of the substrate protein changes its thermal stability, and applies the concept of specificity to reveal the potential substrates. For selenoprotein thioredoxin reductase 1, SIESTA confirmed several known protein substrates and suggested novel candidates. For poly-(ADP-ribose) polymerase-10, SIESTA revealed a number of PARP10 putative substrates, which were confirmed by targeted mass spectrometry and functional assays. Wider application of SIESTA can enhance our understanding of the role of enzymes in homeostasis and disease, and facilitate drug discovery. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:17:42.384.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Amir Ata Saei |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-07-25 02:35:04 | ID requested | |
1 | 2021-01-10 23:48:02 | announced | |
2 | 2021-04-06 02:10:42 | announced | 2021-04-06: Updated publication reference for PubMed record(s): 33637753. |
⏵ 3 | 2024-10-22 05:17:43 | announced | 2024-10-22: Updated project metadata. |
Publication List
Saei AA, Beusch CM, Sabatier P, Wells JA, Gharibi H, Meng Z, Chernobrovkin A, Rodin S, N, ä, reoja K, Thorsell AG, Karlberg T, Cheng Q, Lundstr, ö, m SL, Gaetani M, V, é, gv, á, ri Á, Arn, é, r ESJ, Sch, ü, ler H, Zubarev RA, System-wide identification and prioritization of enzyme substrates by thermal analysis. Nat Commun, 12(1):1296(2021) [pubmed] |
10.1038/s41467-021-21540-6; |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Poly-(ADP-ribose) polymerase-10, post-translational modification, target, thioredoxin reductase 1,ARTD10, thermal stability, TrxR1, PARP10, MARylation, substrate |
Contact List
Roman Zubarev | |
---|---|
contact affiliation | Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden |
contact email | Roman.zubarev@ki.se |
lab head | |
Amir Ata Saei | |
contact affiliation | Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden |
contact email | amirata.saei.dibavar@ki.se |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD010554 |
PRIDE project URI |
Repository Record List
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