Exosomes (EXOs) are a biological subset of extracellular vesicles of 50-200 nm in size which are released by most cells upon fusion of multivehicular bodies with plasma membrane.Mimetic-Nanovesicles (M-NVs) have higher yield compare to exosomes. In addition, M-NVs can untangle the difficulties associated with the isolation and purification of cell culture exosomes. M-NVs is consist of membranouslipid bilayer which could be derived from extrusion of cells through filter membrane. Several studies have used M-NVs for therapeutic delivery of genetic materials and drug. The proteomic content of these vesicles with regard to protein identities and post-translational modifications remain uncharacterized and proteomic data is presented in this dataset.