PXD010537 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phospho and expression proteomics in chinese hamster ovary cells |
Description | We investigated CHO signaling events in a comparative expression and phospho proteomics approach of recombinant mAb producing XL99 cell line and according parental cells. First, on proteomic level differences between producer and parental cells were evaluated by label-free quantification of proteins at exponential growth. Next, a SILAC-based phosphoproteomic investigation was used to elucidate differential phosphorylation events following IGF treatment as well as differences between two producer cell lines and the parental cells. |
HostingRepository | PRIDE |
AnnounceDate | 2020-02-26 |
AnnouncementXML | Submission_2020-02-26_07:30:33.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Louise Brachtvogel |
SpeciesList | scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-07-24 00:00:03 | ID requested | |
⏵ 1 | 2020-02-26 07:30:34 | announced | |
Publication List
Schelletter L, Albaum S, Walter S, Noll T, Hoffrogge R, Clonal variations in CHO IGF signaling investigated by SILAC-based phosphoproteomics and LFQ-MS. Appl Microbiol Biotechnol, 103(19):8127-8143(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: LC-MSMS, SILAC, LFQ, CHO, Phosphoproteomics, Proteomics |
Contact List
Dr. Raimund Hoffrogge |
contact affiliation | Cell culture technology, Technical faculty, Bielefeld University |
contact email | raimund.hoffrogge@uni-bielefeld.de |
lab head | |
Louise Brachtvogel |
contact affiliation | Bielefeld University |
contact email | l.brachtvogel@uni-bielefeld.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010537
- Label: PRIDE project
- Name: Phospho and expression proteomics in chinese hamster ovary cells