PXD010525

PXD010525 is an original dataset announced via ProteomeXchange.

Title	Differential view on the bile acids stress response of Clostridioides difficile_longterm stress
Description	In this study, we aimed at the characterization of C. difficile’s stress response to the main four human bile acids. Although, a phenotypically description of growth differences upon challenge with different bile acids has been described (Lewis 2016, Thanissery 2017), there is no information on the adaptation of gene expression available. We employed a comprehensive proteomics approach to record stress signatures of the unconjugated bile acids CA, CDCA, DCA and LCA during long-term-stress conditions and could depict a general stress response concerning all four bile acids, but also specific responses to only a single or a few of the different bile acids. Our results are a starting point for the understanding of how the individual bile acids cocktail of a patient can decide on the outcome of a C. difficile infection
HostingRepository	PRIDE
AnnounceDate	2019-02-13
AnnouncementXML	Submission_2019-03-11_14:38:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Zuehlke
SpeciesList	scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: 272563;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-22 23:31:05	ID requested
1	2019-02-13 06:00:21	announced
⏵ 2	2019-03-11 14:38:09	announced	Updated publication reference for PubMed record(s): 30833939.

Sievers S, Metzendorf NG, Dittmann S, Troitzsch D, Gast V, Tr, ö, ger SM, Wolff C, Z, ü, hlke D, Hirschfeld C, Schl, ü, ter R, Riedel K, . Front Microbiol, 10():258(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Clostridioides difficile, proteomics, bile acids, motility, flagella, general and specific stress response, differential analysis

Kathrin Riedel
contact affiliation	Department of Microbial Physiology and Molecular Biology, Institute of Microbiology, University of Greifswald
contact email	riedela@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliation	Ernst Moritz Arndt University Greifswald, Institute of Microbiology
contact email	zuehlke@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD010525

PRIDE project URI

• PRIDE
  1. PXD010525
     1. Label: PRIDE project
     2. Name: Differential view on the bile acids stress response of Clostridioides difficile_longterm stress