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PXD010520

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-12-06
  • AnnouncementXML: Submission_2018-12-06_04:21:57.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Sophia Foehr
  • Title: The Human RNA-Binding Proteome and Its Dynamics During Arsenite-Induced Translational Arrest
  • Description: The data presented here was produced using XRNAX - a novel extraction method for protein-crosslinked RNA from UV-crosslinked cells. We apply XRNAX for three proteomic downstream applications. First, we purify ribonucleotide-crosslinked peptides from XRNAX extracts and use their identification as direct evidence for protein-RNA interfaces. Second, we use SILAC, XRNAX and silica enrichment in order to derive high-confidence RNA-binding proteomes from the three human cell lines MCF7, HEK293 and HeLa. Third, using SILAC we quantify total proteomes and RNA-binding proteomes differentially during a time course experiment of arsenite stress in MCF7 cells.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: No PTMs are included in the dataset
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-07-20 07:27:39ID requested
12018-12-06 04:21:58announced

Publication List

  1. Dataset with its publication pending

Keyword List

  1. curator keyword: Biomedical
  2. submitter keyword: XRNAX, protein-crosslinked RNA extraction, UV-crosslinking, RNA-binding proteins, nucleotide-crosslinked peptides, human RNA-binding proteome, arsenite stress, translational arrest, ribophagy

Contact List

    Jeroen Krijgsveld
    • contact affiliation: German Cancer Research Center (DKFZ), Heidelberg, Germany
    • contact email: j.krijgsveld@dkfz-heidelberg.de
    • lab head:
    Sophia Foehr
    • contact affiliation: DKFZ
    • contact email: sophia.foehr@dkfz.de
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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