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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-12-04
  • AnnouncementXML: Submission_2018-12-04_01:41:30.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD010515
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Matthew McIlvin
  • Title: Trichodesmium LC-MSMS iron and phosphorous co-limitation in the ocean
  • Description: Nitrogen fixation by cyanobacteria supplies critical bioavailable nitrogen to marine ecosystems worldwide; however, field and lab data have demonstrated it to be limited by iron, phosphorus and/or CO2. To address unknown future interactions among these factors, we grew the nitrogen-fixing cyanobacterium Trichodesmium for 1 year under Fe/P co-limitation following 7 years of both low and high CO2 selection. Fe/P co-limited cell lines demonstrated a complex cellular response including increased growth rates, broad proteome restructuring and cell size reductions relative to steady-state growth limited by either Fe or P alone. Fe/P co-limitation increased abundance of a protein containing a conserved domain previously implicated in cell size regulation, suggesting a similar role in Trichodesmium. Increased CO2 further induced nutrient-limited proteome shifts in widespread core metabolisms. Our results thus suggest that N2-fixing microbes may be significantly impacted by interactions between elevated CO2 and nutrient limitation, with broad implications for global biogeochemical cycles in the future ocean. Sample number sample description 1 380-1 2 380-2 3 380-3 4 750-1 5 750-2 6 750-3 7 380-1P 8 380-2P 9 380-3P 10 800-1P 11 800-2P 12 800-3P 13 380-1Fe 14 380-2Fe 15 380-3Fe 16 750-1Fe 17 750-2Fe 18 750-3Fe 19 380-1FeP 20 380-2FeP 21 380-3FeP 22 750-1FeP 23 750-2FeP 24 750-3FeP
  • SpeciesList: scientific name: Trichodesmium erythraeum IMS101; NCBI TaxID: 203124;
  • ModificationList: Oxidation; Carbamidomethyl
  • Instrument: Orbitrap Fusion

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-07-20 04:16:25ID requested
12018-08-09 09:40:07announced
22018-12-04 01:41:31announcedUpdated project metadata.

Publication List

  1. Walworth NG, Fu FX, Webb EA, Saito MA, Moran D, Mcllvin MR, Lee MD, Hutchins DA, Mechanisms of increased Trichodesmium fitness under iron and phosphorus co-limitation in the present and future ocean. Nat Commun, 7():12081(2016) [pubmed]

Keyword List

  1. curator keyword: Metaproteomics
  2. submitter keyword: Trichodesmium, ocean marine microbes, iron and phosphorus co-limitation, Thermo Fusion

Contact List

    Mak Saito
    • contact affiliation: Marine Chemistry/Geochemistry Woods Hole Oceanographic Institution USA
    • contact email: msaito@whoi.edu
    • lab head:
    Matthew McIlvin
    • contact affiliation: Woods Hole Oceanographic Inst.
    • contact email: mmcilvin@whoi.edu
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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