PXD010510

PXD010510 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A high throughput pipeline for antibody validation
Description	Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we show that an inverted version of WB allows parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). The pipeline provides means for large-scale implementation of concepts proposed by an international working group on antibody validation (IWGAV).
HostingRepository	PRIDE
AnnounceDate	2018-07-26
AnnouncementXML	Submission_2018-07-26_06:26:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Krzysztof Sikorski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-20 03:49:14	ID requested
⏵ 1	2018-07-26 06:26:27	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

curator keyword: Biomedical
submitter keyword: LC-MS/MS, antibody validation, IP-MS

curator keyword: Biomedical

submitter keyword: LC-MS/MS, antibody validation, IP-MS

Contact List

Fridtjof Lund-Johansen
contact affiliation	Oslo University Hospital, Oslo, Norway
contact email	fridtjol@gmail.com
lab head
Krzysztof Sikorski
contact affiliation	K.G. Jebsen Center for Cancer Immunotherapy, Oslo, Norway
contact email	kristof.sikorski@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD010510
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD010510

PRIDE project URI

Repository Record List

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