PXD010508

PXD010508 is an original dataset announced via ProteomeXchange.

Title	Activated neutrophils exert myeloid-derived suppressor cell activity, damaging T cells beyond repair
Description	Myeloid-derived suppressor cells (MDSCs) have the capacity to suppress T cell-mediated immune responses, and impact clinical outcome of cancer, infections and transplantation settings. Although MDSCs were initially described as bone-marrow-derived immature myeloid cells (either monocytic [m-MDSC] or granulocytic [g-MDSC]), also mature neutrophils have been shown to exert MDSC activity towards T cells, in ways that so far remained unclear. In this study, we demonstrate that human neutrophils – both from healthy donors and cancer patients – do not exert MDSC activity unless they are activated. Using neutrophils with genetically well-defined defects, we found that reactive oxygen species (ROS) and granule-derived constituents are required for MDSC activity after direct CD11b-dependent neutrophil-T cell interactions. Besides these cellular interactions, neutrophils were engaged in the uptake of pieces of T cell membrane, a process called trogocytosis. Together, these interactions led to changes in T cell morphology, mitochondrial dysfunction and ATP depletion, as indicated by electron microscopy, mass spectrometry and metabolic parameters. Our studies characterize the different steps by which activated mature neutrophils induce functional T cell non-responsiveness and irreparable cell damage.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:03:22.358.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cathelijn Aarts
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-20 01:13:37	ID requested
1	2020-05-07 04:14:10	announced
⏵ 2	2024-10-22 05:03:27	announced	2024-10-22: Updated project metadata.

Aarts CEM, Hiemstra IH, B, é, guin EP, Hoogendijk AJ, Bouchmal S, van Houdt M, Tool ATJ, Mul E, Jansen MH, Janssen H, van Alphen FPJ, de Boer JP, Zuur CL, Meijer AB, van den Berg TK, Kuijpers TW, Activated neutrophils exert myeloid-derived suppressor cell activity damaging T cells beyond repair. Blood Adv, 3(22):3562-3574(2019) [pubmed]

10.1182/bloodadvances.2019031609;

curator keyword: Biomedical

submitter keyword: large T cells,MDSC, small T cells

T.W. Kuijpers
contact affiliation	Department of Pediatric Hematology, Immunology & Infectious Disease, Emma Children’s Hospital, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
contact email	t.w.kuijpers@amc.uva.nl
lab head
Cathelijn Aarts
contact affiliation	Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, AMC, University of Amsterdam, Amsterdam, The Netherlands
contact email	c.aarts@sanquin.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD010508

PRIDE project URI

• PRIDE
  1. PXD010508
     1. Label: PRIDE project
     2. Name: Activated neutrophils exert myeloid-derived suppressor cell activity, damaging T cells beyond repair