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PXD010508

PXD010508 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleActivated neutrophils exert myeloid-derived suppressor cell activity, damaging T cells beyond repair
DescriptionMyeloid-derived suppressor cells (MDSCs) have the capacity to suppress T cell-mediated immune responses, and impact clinical outcome of cancer, infections and transplantation settings. Although MDSCs were initially described as bone-marrow-derived immature myeloid cells (either monocytic [m-MDSC] or granulocytic [g-MDSC]), also mature neutrophils have been shown to exert MDSC activity towards T cells, in ways that so far remained unclear. In this study, we demonstrate that human neutrophils – both from healthy donors and cancer patients – do not exert MDSC activity unless they are activated. Using neutrophils with genetically well-defined defects, we found that reactive oxygen species (ROS) and granule-derived constituents are required for MDSC activity after direct CD11b-dependent neutrophil-T cell interactions. Besides these cellular interactions, neutrophils were engaged in the uptake of pieces of T cell membrane, a process called trogocytosis. Together, these interactions led to changes in T cell morphology, mitochondrial dysfunction and ATP depletion, as indicated by electron microscopy, mass spectrometry and metabolic parameters. Our studies characterize the different steps by which activated mature neutrophils induce functional T cell non-responsiveness and irreparable cell damage.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:03:22.358.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCathelijn Aarts
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-20 01:13:37ID requested
12020-05-07 04:14:10announced
22024-10-22 05:03:27announced2024-10-22: Updated project metadata.
Publication List
Aarts CEM, Hiemstra IH, B, é, guin EP, Hoogendijk AJ, Bouchmal S, van Houdt M, Tool ATJ, Mul E, Jansen MH, Janssen H, van Alphen FPJ, de Boer JP, Zuur CL, Meijer AB, van den Berg TK, Kuijpers TW, Activated neutrophils exert myeloid-derived suppressor cell activity damaging T cells beyond repair. Blood Adv, 3(22):3562-3574(2019) [pubmed]
10.1182/bloodadvances.2019031609;
Keyword List
curator keyword: Biomedical
submitter keyword: large T cells,MDSC, small T cells
Contact List
T.W. Kuijpers
contact affiliationDepartment of Pediatric Hematology, Immunology & Infectious Disease, Emma Children’s Hospital, Academic Medical Center (AMC), University of Amsterdam, Amsterdam, The Netherlands
contact emailt.w.kuijpers@amc.uva.nl
lab head
Cathelijn Aarts
contact affiliationDepartment of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, AMC, University of Amsterdam, Amsterdam, The Netherlands
contact emailc.aarts@sanquin.nl
dataset submitter
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Dataset FTP location
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PRIDE project URI
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