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PXD010506

PXD010506 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrated Transcriptomics, Proteomics and Glycomics for a Deeper Insight of the Mechanism of Breast Cancer Brain Metastasis: Emphasizing on Glycosylation
DescriptionBreast cancer brain metastasis has been recognized as one of the central issues in breast cancer research. Elucidation of the process and pathway that mediate this step is expected to provide important clues for a better understanding of breast cancer metastasis. Increasing evidence suggests that the aberrant glycosylation patterns greatly contribute to the cell invasion and cancer metastasis. Herein, we combined next generation RNA sequencing with liquid chromatograph-tandem mass spectrometry based proteomic and N-glycomic analysis from five breast cancer cell lines and one brain cancer cell line to investigate the possible mechanism of breast cancer brain metastasis. 24763 genes were identified including 14551 differentially expressed genes across six cell lines while proteomic analysis allowed the quantitation of 1096 differentially expressed proteins with approximately 83.8% proteins’ regulation matching their gene expression change. The genes/proteins associated with cell movement were highlighted in the breast cancer brain metastasis. Integrin signaling pathway and the up-regulation of α-integrin (ITGA2, ITGA3) associated with the brain metastatic process was shown through Ingenuity Pathway Analysis (IPA). Overall 91 glycosylation genes were selected from transcriptomic data and all exhibited differential expression. 12 glycogenes showed unique expression in 231BR. The regulation of these genes could result in an activation prediction of sialylation function in 231BR by ingenuity pathway analysis. In agreement with the changes of glycogenes, 60 N-glycans out of 63 identified exhibited differential expression among cell lines. The correlation of glycogenes and glycans revealed the importance of sialylation and sialylated glycans in breast cancer brain metastasis. Highly sialylated glycans, which were up-regulated in brain seeking cell line 231BR, probably contributes to brain metastasis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:04:46.201.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWenjing Peng
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-20 00:56:59ID requested
12020-05-26 12:04:28announced
22024-10-22 05:04:49announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41598-019-53984-8;
Peng W, Zhu R, Zhou S, Mirzaei P, Mechref Y, Integrated Transcriptomics, Proteomics, and Glycomics Reveals the Association between Up-regulation of Sialylated N-glycans/Integrin and Breast Cancer Brain Metastasis. Sci Rep, 9(1):17361(2019) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Integrative omics analysis, Glycomics, Transcriptomics, Proteomics,Breast cancer brain metastasis
Contact List
Yehia Mechref
contact affiliationDepartment of chemistry and biochemistry, Texas Tech University, Lubbock, Texas, USA, 79413.
contact emailyehia.mechref@ttu.edu
lab head
Wenjing Peng
contact affiliationTexas Tech University
contact emailwenjing.peng@ttu.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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