PXD010505

PXD010505 is an original dataset announced via ProteomeXchange.

Title	Serum resistance of Neisseria meningitidis strains of the ST-41/44 clonal complex is influenced by a homopolymeric adenosine tract in the promotor region of the nspA gene
Description	Although usually a harmless colonizer of the human nasopharynx, Neisseria meningitidis (meningococcus) can spread to the blood stream and cause invasive disease. For survival in blood, N. meningitidis evades the complement system by expression of a polysaccharide capsule and surface proteins sequestering the complement regulator fH. Meningococcal strains are highly diverse and are categorized by their serogroup and multilocus sequence typing. The sequence type 41/44 clonal complex makes up a major proportion of serogroup B meningococcal disease worldwide, but it is also common in asymptomatic carriers. Proteome analysis of a serum resistant isolate from invasive meningococcal disease and two less resistant isolates from healthy carriers identified NspA as the sole protein consistently expressed more abundantly in the invasive isolate. Knock-out of nspA reduced serum resistance, accompanied by stronger deposition of membrane attack complex (C5b9). High or low expression of NspA was associated with sequence variation within a homopolymeric tract located in the -10/-35 region of the nspA promotor: A tract with 5 adenosines dictated low NspA expression, whereas a 6-adenosine motif led to high NspA expression. High levels of NspA correlated with high factor H sequestration onto the bacteria. We could not link the homopolymeric tract length to phase variation, unlike described for other N. meningitidis surface proteins with similar sequence motifs. Epidemiological evidence from carriage and disease isolates indicates that NspA contributes to serum resistance, but is not a prerequisite for invasive disease. Thus, the lineage ST-41/44 meningococcal strains are heterogenous in their NspA expression.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:49.104.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Otto
SpeciesList	scientific name: Neisseria meningitidis; NCBI TaxID: 487;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-20 00:14:50	ID requested
1	2019-02-28 04:35:25	announced
⏵ 2	2024-10-22 04:46:49	announced	2024-10-22: Updated project metadata.

10.1038/s41598-019-39231-0;

Claus H, Hubert K, Becher D, Otto A, Pawlik MC, Lappann I, Strobel L, Vogel U, Johswich K, A homopolymeric adenosine tract in the promoter region of nspA influences factor H-mediated serum resistance in Neisseria meningitidis. Sci Rep, 9(1):2736(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Neisseria meningitidis, serum resistance, NspA, ST-41/44

Prof. Dr. Becher
contact affiliation	Institut für Mikrobiologie Dept. Mikrobielle Proteomik Universität Greifswald Felix-Hausdorff-Str. 8 17489 Greifswald
contact email	dbeche@uni-greifswald.de
lab head
Andreas Otto
contact affiliation	Institute for Microbiology
contact email	andreas.otto@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD010505

PRIDE project URI

• PRIDE
  1. PXD010505
     1. Label: PRIDE project
     2. Name: Serum resistance of Neisseria meningitidis strains of the ST-41/44 clonal complex is influenced by a homopolymeric adenosine tract in the promotor region of the nspA gene