PXD010495

PXD010495 is an original dataset announced via ProteomeXchange.

Title	Effect of purification of galactooligosaccharides derived from lactulose with Saccharomyces cerevisiae on their capacity to bind immune cell receptor Dectin-2
Description	Lactulose-derived oligosaccharides (OsLu) are prebiotic galactooligosaccharides (GOS) known to be beneficial for human health. Many of these carbohydrates are reported to have immunomodulatory properties; however the molecular mechanism is unclear. OsLu produced by enzymatic synthesis can be purified with Saccharomyces cerevisiae (OsLu-Sc). We show that this purification method introduces yeast-derived proteins reactive to Dectin-2, an innate immune receptor for fungal polysaccharides. Using a cell-based bioassay, we tested the binding of OsLu and GOS samples to Dectin-2. While OsLu purified with active charcoal and commercial GOS failed to bind to Dectin-2, we found OsLu-Sc bound to this receptor. The carbohydrate-binding incompetent mutant of Dectin-2 failed to bind to OsLu-Sc. These data suggest that OsLu-Sc introduced carbohydrate ligands for Dectin-2. In accordance with this, proteomic analysis revealed OsLu-Sc contained S. cerevisiae-derived mannoproteins. Therefore, our data highlights the importance of the purification method for OsLu, which may positively affect the bioactivity of OsLu.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:32.493.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010495
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sergio Ciordia
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	Methylthio; Glu->pyro-Glu; Oxidation; Acetyl; Gln->pyro-Glu
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-19 02:09:44	ID requested
1	2018-12-11 02:43:57	announced
2	2019-02-12 07:37:54	announced	Updated publication reference for PubMed record(s): 30599920.
⏵ 3	2024-10-22 04:46:37	announced	2024-10-22: Updated project metadata.

Young ID, Montilla A, Olano A, Wittmann A, Kawasaki N, Villamiel M, Effect of purification of galactooligosaccharides derived from lactulose with Saccharomyces cerevisiae on their capacity to bind immune cell receptor Dectin-2. Food Res Int, 115():10-15(2019) [pubmed]

10.6019/PXD010495;

10.1016/j.foodres.2018.07.039;

curator keyword: Biological

submitter keyword: Saccharomyces, proteomics, oligosaccharides, mannoproteins,prebiotics, lactulose, Dectin-2

Mar Villamiel Guerra
contact affiliation	Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM)
contact email	m.villamiel@csic.es
lab head
Sergio Ciordia
contact affiliation	Spanish National Center for Biotechnology
contact email	sciordia@cnb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010495
     1. Label: PRIDE project
     2. Name: Effect of purification of galactooligosaccharides derived from lactulose with Saccharomyces cerevisiae on their capacity to bind immune cell receptor Dectin-2