PXD010489

PXD010489 is an original dataset announced via ProteomeXchange.

Title	Muscle proteomics of mitochondrial disorders
Description	Mitochondrial DNA mutations progressively compromise the respiratory chain of skeletal muscle, resulting in a mosaic of metabolically healthy and defective fibers. This heterogeneity is the best diagnostic signpost of mitochondrial disorders. However, the single fiber investigation of its molecular basis has been beyond the capability of large-scale technologies so far. We here use a novel approach to single muscle fibers proteomics, based on laser capture microdissection to excise thin sections of individual muscle fibers from frozen biopsies. Combined with a highly sensitive mass spectrometry-based proteomics workflow, this technique allowed us to analyze healthy and defective muscle fibers from patients with mitochondrial disorders.
HostingRepository	PRIDE
AnnounceDate	2019-11-15
AnnouncementXML	Submission_2020-01-14_06:41:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marta Murgia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-19 01:31:06	ID requested
1	2019-11-15 06:25:28	announced
⏵ 2	2020-01-14 06:41:25	announced	2020-01-14: Updated publication reference for PubMed record(s): 31851916.

Murgia M, Tan J, Geyer PE, Doll S, Mann M, Klopstock T, Proteomics of Cytochrome c Oxidase-Negative versus -Positive Muscle Fiber Sections in Mitochondrial Myopathy. Cell Rep, 29(12):3825-3834.e4(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: human muscle, Q Exactive HF, single muscle fibers, mitochondrial disorders, laser microdissection,

Thomas Klopstock
contact affiliation	Friedrich-Baur-Institute, Dept. of Neurology, University of Munich, 80336 Munich, Germany
contact email	Thomas.Klopstock@med.uni-muenchen.de
lab head
Marta Murgia
contact affiliation	MPI Biochemistry
contact email	mmurgia@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD010489

PRIDE project URI

• PRIDE
  1. PXD010489
     1. Label: PRIDE project
     2. Name: Muscle proteomics of mitochondrial disorders