PXD010476

PXD010476 is an original dataset announced via ProteomeXchange.

Title	The natural product elegaphenone potentiates antibiotic effects against Pseudomonas aeruginosa
Description	Natural products represent a rich source for antibiotics addressing versatile cellular targets. The deconvolution of their targets via chemical proteomics is often challenged by the introduction of large photocrosslinkers. Here we select elegaphenone, a largely uncharacterized natural product antibiotic bearing a native benzophenone core scaffold, for affinity-based protein profiling (AfBPP) in Gram-positive and Gram-negative bacteria. This study utilizes the alkynylated natural product scaffold as a probe to uncover intriguing biological interactions with the transcriptional regulator AlgP. Furthermore, proteome profiling of a Pseudomonas aeruginosa AlgP transposon mutant revealed unique insights into the mode of action. Elegaphenone enhanced the killing of intracellular P. aeruginosa in macrophages exposed to sub-inhibitory concentrations of the fluoroquinolone antibiotic norfloxacin.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_07:26:47.603.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weining Zhao
SpeciesList	scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-18 02:57:50	ID requested
⏵ 1	2021-09-08 07:26:48	announced

Zhao W, Cross AR, Crowe-McAuliffe C, Weigert-Munoz A, Csatary EE, Solinski AE, Krysiak J, Goldberg JB, Wilson DN, Medina E, Wuest WM, Sieber SA, The Natural Product Elegaphenone Potentiates Antibiotic Effects against Pseudomonas aeruginosa. Angew Chem Int Ed Engl, 58(25):8581-8584(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: antimicrobials, antivirulence, chemical proteomics, natural products

Stephan Axel Sieber
contact affiliation	Technische Universität München, Chair of Organic Chemistry,Garching, Germany
contact email	stephan.sieber@tum.de
lab head
Weining Zhao
contact affiliation	TU Munich Chair of Organic Chemistry 2 Lichtenbergstraße 4 85748 Garching Germany
contact email	weining.zhaotum@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010476
     1. Label: PRIDE project
     2. Name: The natural product elegaphenone potentiates antibiotic effects against Pseudomonas aeruginosa