Gonadotrophins, including Human chorionic gonadotrophin (hCG), have been used since and for several decades to treat infertility by ovarian stimulation. The hCG is being extracted from urine of pregnant women and it does inevitably contains other proteins secreted into urine. The presence of other proteins varies from batch to batch and it can be significantly high. Current study investigated the presence of proteins other than hCG in different batches of commercial formulations produced by extraction from urine and by using the recombinant approach. The total protein content varied from batch to batch and a large number of contaminant urinary proteins were identified in all analyzed samples except for the recombinant product