PXD010470

PXD010470 is an original dataset announced via ProteomeXchange.

Title	SseF-SscB Interaction
Description	Bacterial type III secretion systems are able to secrete membrane proteins. In this project we could show that cognate T3SS chaperones are able to bind to the transmembrane segments of T3SS substrates to avoid erroneous targeting to the bacterial inner membrane. Here, we studied the Salmonella SPI-2 T3SS secreted protein SseF and the interaction with its chaperone SscB by in vivo photocrosslinking. LC-MS/MS analysis was done to provide additional evidence that SscB is the interaction partner leading to the upshift of the SseF band in an SDS-PAGE analysis.
HostingRepository	PRIDE
AnnounceDate	2018-10-16
AnnouncementXML	Submission_2018-10-16_00:02:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Nalpas
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Typhimurium; NCBI TaxID: 90371;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-18 02:21:53	ID requested
⏵ 1	2018-10-16 00:02:41	announced

Krampen L, Malmsheimer S, Grin I, Trunk T, L, ü, hrmann A, de Gier JW, Wagner S, Revealing the mechanisms of membrane protein export by virulence-associated bacterial secretion systems. Nat Commun, 9(1):3467(2018) [pubmed]

curator keyword: Biological

submitter keyword: Salmonella, Type III secretion systems, pBpa, in vivo photocrosslinking, SseF, SscB

Samuel Wagner
contact affiliation	Sektion of Cellular and Molecular Microbiology Interfaculty Institute of Microbiology and Infection Medicine University of Tübingen Germany
contact email	samuel.wagner@med.uni-tuebingen.de
lab head
Nicolas Nalpas
contact affiliation	Tuebingen University
contact email	nalpas.nicolas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010470

PRIDE project URI

• PRIDE
  1. PXD010470
     1. Label: PRIDE project
     2. Name: SseF-SscB Interaction