PXD010466

PXD010466 is an original dataset announced via ProteomeXchange.

Title	Characterization of legumain degradation of intact proteins
Description	Legumain or asparagine endopeptidase is a unique cysteine endopeptidase with a distinctive specificity for the hydrolysis of peptide bonds after asparagine and to a lesser extent after aspartate. In order to improve our understanding of legumain substrate recognition we have performed proteomic profiling of legumain specificity on native proteins using two different N-terminal labelling methodologies (FPPS and ISIL). Our data revealed a strict P1 specificity of legumain with clear cleavage preference for unstructured secondary regions in the substrate proteins. No extended cleavage specificity on native proteins was observed. Moreover, a limited number of identified cleavages on individual substrates suggest its primary role in precision proteolysis and regulatory proteolytic events.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:56:26.052.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marko Fonovic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-18 01:20:37	ID requested
1	2018-11-29 02:42:27	announced
2	2018-12-04 01:40:36	announced	Updated project metadata.
3	2019-03-11 14:34:24	announced	Updated project metadata.
⏵ 4	2023-11-14 06:56:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: degradome, proteases, legumain, cleavage specificity,proteolysis

Marko Fonovic
contact affiliation	Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia
contact email	marko.fonovic@ijs.si
lab head
Marko Fonovic
contact affiliation	Jozef Stefan Institute
contact email	marko.fonovic@ijs.si
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010466

PRIDE project URI

• PRIDE
  1. PXD010466
     1. Label: PRIDE project
     2. Name: Characterization of legumain degradation of intact proteins