PXD010456

PXD010456 is an original dataset announced via ProteomeXchange.

Title	Sialo-glycoproteome of Human lung cancer cell by LC-MSMS
Description	Alterations in protein glycosylation, especially the terminal sialylation, are closely correlated with physiological and pathological regulation. Due to the low ionization efficiency of sialo-glycopeptides and frequently observed dissociation of sialic acid residues during mass spectrometry analysis, tools to enrich the sialo-glycopeptides are essential to enhance detection sensitivity and identification efficiency in sialo-glycoproteomics. In this study, we present the first application of zwitterionic hydrophilic interaction chromatography (ZIC-cHILIC) material in StageTip for simultaneous enrichment and fractionation of intact glycopeptides at proteome scale. With the demonstrated enrichment specificity and identification depth, the stepwise-ZIC-cHILIC can be an efficient enrichment method for the discovery of glycosylation sites and native glycotope for many sample types.
HostingRepository	PRIDE
AnnounceDate	2022-02-25
AnnouncementXML	Submission_2022-02-24_17:37:51.034.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010456
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yi-Ju Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Deamidated; Oxidation; monohydroxylated residue; deaminated residue; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-17 04:14:23	ID requested
⏵ 1	2022-02-24 17:37:51	announced

Chen YJ, Yen TC, Lin YH, Chen YL, Khoo KH, Chen YJ, ZIC-cHILIC-Based StageTip for Simultaneous Glycopeptide Enrichment and Fractionation toward Large-Scale N-Sialoglycoproteomics. Anal Chem, 93(48):15931-15940(2021) [pubmed]

submitter keyword: glycoproteome, sialo-glycoproteome, human lung cancer

Yu-Ju Chen
contact affiliation	Institute of Chemistry, Academia Sinica
contact email	yujuchen@gate.sinica.edu.tw
lab head
Yi-Ju Chen
contact affiliation	Academia Sinica
contact email	tp7249@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010456
     1. Label: PRIDE project
     2. Name: Sialo-glycoproteome of Human lung cancer cell by LC-MSMS