PXD010455

PXD010455 is an original dataset announced via ProteomeXchange.

Title	Protein and metabolite composition of the Arabidopsis stress granules.
Description	Stress granules are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Herein, we report protein composition of the Arabidopsis SGs supporting their role in plant response to stress. Moreover, and to our knowledge for the first time, we provide evidence for not only mRNAs and proteins, but also phospholipids and amino-acids accumulation in the SGs. A quarter of the identified proteins constituted known or predicted SGs components, supporting evolutionary conserved mechanism of SGs assembly and dynamics. Intriguingly, remaining proteins were enriched in key enzymes and regulators mediating plant response to stress, such as cyclin dependent kinase A (CDKA), pointing to the important role of SGs in moderating plant response to stress by selective storage and temporary inactivation of the proteins. We hypothesize that phospholipid sequestration into SGs may serve partly analogous role providing protection from the phospholipase mediated degradation occurring upon combination of heat and dark stress.
HostingRepository	PRIDE
AnnounceDate	2022-03-27
AnnouncementXML	Submission_2022-03-27_05:44:08.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michal Gorka
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-17 04:06:02	ID requested
⏵ 1	2022-03-27 05:44:09	announced

Kosmacz M, Gorka M, Schmidt S, Luzarowski M, Moreno JC, Szlachetko J, Leniak E, Sokolowska EM, Sofroni K, Schnittger A, Skirycz A, Protein and metabolite composition of Arabidopsis stress granules. New Phytol, 222(3):1420-1433(2019) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis Thaliana, stress granules, protein, metabolite, LC-MS/MS, AP-MS

Dr. Aleksandra Skirycz
contact affiliation	Department Willmitzer | Small-Molecule Signaling Max-Planck-Institut für Molekulare Pflanzenphysiologie Wissenschaftspark Golm Am Mühlenberg 1 14476 Potsdam
contact email	skirycz@mpimp-golm.mpg.de
lab head
Michal Gorka
contact affiliation	Max Planck Institute for Molecular Plant Physiology
contact email	gorka@mpimp-golm.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD010455

PRIDE project URI

• PRIDE
  1. PXD010455
     1. Label: PRIDE project
     2. Name: Protein and metabolite composition of the Arabidopsis stress granules.