PXD010450

PXD010450 is an original dataset announced via ProteomeXchange.

Title	The HLA ligandome landscape of chronic myeloid leukemia delineates novel T-cell epitopes for immunotherapy
Description	Anti-leukemia immunity plays an important role for disease control and maintenance of tyrosine kinase inhibitor (TKI)-free remission in chronic myeloid leukemia (CML). Antigen-specific immunotherapy holds promise to strengthen immune control in CML, but requires the identification of CML-associated targets. In this study, we used a mass spectrometry-based approach to identify naturally presented HLA class I- and class II-presented peptides in 20 primary CML samples. Comparative HLA ligandome profiling using a comprehensive dataset of different hematological benign specimen delineated a novel panel of frequently expressed CML-exclusive peptides. These non-mutated target antigens are of particular relevance since our extensive data-mining approach demonstrated absence of naturally presented BCR-ABL- and ABL-BCR-derived HLA-presented peptides and the lack of frequent tumor-exclusive presentation of predescribed cancer/testis and leukemia-associated antigens. Functional characterization revealed spontaneous T-cell responses against the newly identified CML-associated peptides in CML patients and their ability to de novo induce multifunctional and cytotoxic antigen-specific T cells in healthy volunteers and CML patients. This characterizes these antigens as prime candidates for T cell-based immunotherapy approaches that may prolong TKI-free survival and even mediate cure of CML patients.
HostingRepository	PRIDE
AnnounceDate	2018-12-11
AnnouncementXML	Submission_2018-12-13_00:49:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annika Nelde
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-17 00:51:01	ID requested
1	2018-12-11 00:10:26	announced
⏵ 2	2018-12-13 00:49:13	announced	Updated publication reference for PubMed record(s): 30530751.

Bilich T, Nelde A, Bichmann L, Roerden M, Salih HR, Kowalewski DJ, Schuster H, Tsou CC, Marcu A, Neidert MC, L, ü, bke M, Rieth J, Schemionek M, Br, ü, mmendorf TH, Vucinic V, Niederwieser D, Bauer J, M, ä, rklin M, Peper JK, Klein R, Kohlbacher O, Kanz L, Rammensee HG, Stevanovi, ć S, Walz JS, The HLA ligandome landscape of chronic myeloid leukemia delineates novel T-cell epitopes for immunotherapy. Blood, 133(6):550-565(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: chronic myeloid leukemia, mass spectrometry, immunopeptidome

Juliane S. Walz
contact affiliation	Department of Hematology and Oncology, University of Tübingen, Germany
contact email	juliane.walz@med.uni-tuebingen.de
lab head
Annika Nelde
contact affiliation	Department of Hematology and Oncology, University of Tübingen, Germany and Institute for Cell Biology, Department of Immunology, University of Tübingen, Germany
contact email	annika.nelde@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD010450

PRIDE project URI

• PRIDE
  1. PXD010450
     1. Label: PRIDE project
     2. Name: The HLA ligandome landscape of chronic myeloid leukemia delineates novel T-cell epitopes for immunotherapy