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PXD010444

PXD010444 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleList_MAPS - Human listeriosis cases are due to the ingestion of contaminated foods with the pathogenic bacteria Listeria monocytogenes
DescriptionHuman listeriosis cases are due to the ingestion of contaminated foods with the pathogenic bacteria Listeria monocytogenes. The reduction of water availability in food workshops by decreasing the air relative humidity (RH) is one strategy to improve the control of bacterial contamination. This study aims to develop and implement an MSI approach on L. monocytogenes biofilms and proof of concept using a dehumidified stress condition. MSI allowed examining the distribution of low molecular weight proteins within the biofilms subjected to a dehumidification environment, mimicking the one present in a food workshop (10??C, 75% RH). Furthermore, a LC-MS/MS approach was made to link the dots between MSI and protein identification. Five identified proteins were assigned to registered MSI m/z, including two cold-shock proteins and a ligase involved in cell wall biogenesis.
HostingRepositoryPRIDE
AnnounceDate2020-03-12
AnnouncementXMLSubmission_2020-03-12_03:44:49.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD010444
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTiago Santos
SpeciesList scientific name: Listeria monocytogenes EGD-e; NCBI TaxID: 169963;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-16 08:54:18ID requested
12018-07-30 02:14:30announced
22020-03-12 03:44:51announced2020-03-12: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: Listeria monocytogenes, biofilm, desiccation, LC-MS/MS
Contact List
Michel H??braud
contact affiliationUniversit?? Clermont Auvergne, INRA, UMR MEDiS, F-63122 Saint-Gen??s Champanelle, France. INRA, Plateforme d???Exploration du M??tabolisme, composante prot??omique (PFEMcp), F-63122 Saint-Gen??s Champanelle, France
contact emailmichel.hebraud@inra.fr
lab head
Tiago Santos
contact affiliationUCA, INRA, UMR MEDiS (Saint-Gen??s Champanelle, France)
contact emailtiago.santos@inra.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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